Mouse anti-Mouse Fer Monoclonal Antibody | anti-Fer antibody

Fer Antibody

Cat. Num. AAA9200057

• Gene Names: Fer; Fer; Fert; Fert2; AV082135; C330004K01Rik
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified Mouse Monoclonal Antibody (Mab)
• Synonyms: Fer; Monoclonal Antibody; Fer Antibody; Tyrosine-protein kinase Fer; Proto-oncogene c-Fer; p94-Fer; Fert2; anti-Fer antibody

• Host: Mouse
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1487CT794.8.50
• Specificity: This Fer antibody is generated from a mouse immunized with a recombinant protein.
• Purity/Purification: Purified Mouse Monoclonal Antibody (Mab)
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 822

• Applicable Applications for anti-Fer antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:4000
• Crown Antibody: Yes
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-Fer Antibody at 1:4000 dilutionLane 1: NIH/3T3 whole cell lysatesLane 2: mouse testis lysatesLane 3: mouse liver lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 95 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-Fer antibody

Tyrosine-protein kinase that acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. Acts downstream of EGFR, KIT, PDGFRA and PDGFRB. Acts downstream of EGFR to promote activation of NF-kappa-B and cell proliferation. May play a role in the regulation of the mitotic cell cycle. Plays a role in the insulin receptor signaling pathway and in activation of phosphatidylinositol 3-kinase. Acts downstream of the activated FCER1 receptor and plays a role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Plays a role in the regulation of mast cell degranulation. Plays a role in leukocyte recruitment and diapedesis in response to bacterial lipopolysaccharide (LPS). Phosphorylates CTTN, CTNND1, PTK2/FAK1, GAB1, PECAM1 and PTPN11. May phosphorylate JUP and PTPN1. Can phosphorylate STAT3 according to PubMed:10878010 and PubMed:19159681, but clearly plays a redundant role in STAT3 phosphorylation. According to PubMed:11134346, cells where wild type FER has been replaced by a kinase-dead mutant show no reduction in STAT3 phosphorylation. Phosphorylates TMF1. Isoform 3 lacks kinase activity.

Product Categories/Family for anti-Fer antibody

Crown Antibodies

References

Letwin K.,et al.Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases.
Fischman K.,et al.Mol. Cell. Biol. 10:146-153(1990).
Iwanishi M.,et al.J. Biol. Chem. 275:38995-39000(2000).
Carninci P.,et al.Science 309:1559-1563(2005).
Kim L.,et al.Mol. Cell. Biol. 15:4553-4561(1995).

NCBI and Uniprot Product Information

• NCBI GI #: 226054043
• NCBI GeneID: 14158
• NCBI Accession #: NP_001033086.2
• NCBI GenBank Nucleotide #: NM_001037997.3
• UniProt Accession #: P70451; Q61561; Q6PEE5; Q80UI3; Q8C481; Q9EQ77
• Molecular Weight: 94579
• NCBI Official Full Name: tyrosine-protein kinase Fer isoform a
• NCBI Official Synonym Full Names: fer (fms/fps related) protein kinase
• NCBI Official Symbol: Fer
• NCBI Official Synonym Symbols: Fer; Fert; Fert2; AV082135; C330004K01Rik
• NCBI Protein Information: tyrosine-protein kinase Fer
• UniProt Protein Name: Tyrosine-protein kinase Fer
• Protein Family: Ferritin
• UniProt Gene Name: Fer
• UniProt Synonym Gene Names: Fert2
• UniProt Entry Name: FER_MOUSE

Uniprot Description

Fer iso4: a non-receptor tyrosine kinase of the Fer family. Regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. Increased expression associated with prostate cancer proliferation, and antisense blocks soft agar growth.

Protein type: EC 2.7.10.2; Kinase, protein; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Oncoprotein

Cellular Component: microtubule cytoskeleton; extrinsic to internal side of plasma membrane; cell projection; cytoskeleton; membrane; lamellipodium; nuclear chromatin; cytoplasm; plasma membrane; cell junction; nucleus; actin cytoskeleton

Molecular Function: nucleotide binding; non-membrane spanning protein tyrosine kinase activity; actin binding; protein phosphatase 1 binding; protein kinase binding; protein kinase activity; transferase activity; protein binding; cadherin binding; protein-tyrosine kinase activity; gamma-catenin binding; cytoskeletal protein binding; transferase activity, transferring phosphorus-containing groups; kinase activity; cell adhesion molecule binding; lipid binding; ATP binding; Rab GTPase binding; epidermal growth factor receptor binding; receptor binding

Biological Process: regulation of mast cell degranulation; peptidyl-tyrosine phosphorylation; diapedesis; protein amino acid autophosphorylation; tyrosine phosphorylation of Stat3 protein; response to lipopolysaccharide; negative regulation of mast cell activation during immune response; platelet-derived growth factor receptor signaling pathway; chemotaxis; signal transduction; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; cell adhesion; epidermal growth factor receptor signaling pathway; regulation of protein amino acid phosphorylation; cytokine and chemokine mediated signaling pathway; microtubule cytoskeleton organization and biogenesis; regulation of epidermal growth factor receptor signaling pathway; regulation of cell proliferation; cell proliferation; positive regulation of actin filament polymerization; cellular response to insulin stimulus; actin cytoskeleton reorganization; innate immune response; mitotic cell cycle; phosphorylation; positive regulation of cell migration