Mouse Fen-1 Monoclonal Antibody | anti-Fen1 antibody

FEN-1 Monoclonal Antibody

Cat. Num. AAA190158

• Gene Names: Fen1; AW538437
• Applications: Immunoblot, Western Blot
• Synonyms: Fen-1; Monoclonal Antibody; FEN-1 Monoclonal Antibody; Anti-Fen-1; FEN-1; anti-Fen1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Specificity: Anti-Fen-1 [Murine MAb to an epitope within aa 1-380 of human FEN-1]; This antibody recognizes human FEN-1.
• Form/Format: 100 ug in 10 mM PBS, pH 7.4.

• Applicable Applications for anti-Fen1 antibody: Immunoblot
• Application Notes: Immunoblotting: use at 1-5 ug/ml.; Positive controls: MCF-7 cells and recombinant fusion protein.
• Antigen: Recombinant human FEN-1 protein (aa 1-380) purified from baculovirus-infected Sf-9 cells.
• Stabilizer: None
• Preservatives: None. Available on request
• Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.

Related Product Information for anti-Fen1 antibody

FEN-1 (Flap Endonuclease and Five Prime Exonuclease) is a 42 kD protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 appears to have important roles in DNA replication and repair and is required in cell-fre DNA replication systems.

Product Categories/Family for anti-Fen1 antibody

Neuroscience and Signal Transduction Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 46048490
• NCBI GeneID: 14156
• NCBI Accession #: NP_032025.2
• NCBI GenBank Nucleotide #: NM_007999.3
• UniProt Accession #: Q3TGH6; P39749; Q8C5X6; Q8C952; Q8R069; Q91Z50; Q99M86
• Molecular Weight: 42,565 Da
• NCBI Official Full Name: flap endonuclease 1
• NCBI Official Synonym Full Names: flap structure specific endonuclease 1
• NCBI Official Symbol: Fen1
• NCBI Official Synonym Symbols: AW538437
• NCBI Protein Information: flap endonuclease 1; FEN-1; OTTMUSP00000046515; OTTMUSP00000046517; OTTMUSP00000046518; flap structure-specific endonuclease 1
• UniProt Protein Name: Putative uncharacterized protein
• UniProt Gene Name: Fen1
• UniProt Entry Name: Q3TGH6_MOUSE

Uniprot Description

FEN1: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. Interacts with PCNA. Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity. The C-terminal domain binds EP300. Can bind simultaneously to both PCNA and EP300. Interacts with DDX11. Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.

Protein type: EC 3.1.-.-; Ribonuclease; DNA repair, damage; DNA-binding; Nucleolus; Nuclear receptor co-regulator; Deoxyribonuclease

Cellular Component: mitochondrion; membrane; nucleolus; plasma membrane; nucleus

Molecular Function: nuclease activity; hydrolase activity; 5'-flap endonuclease activity; metal ion binding; magnesium ion binding; hydrolase activity, acting on ester bonds; T/G mismatch-specific endonuclease activity; flap endonuclease activity; phosphoric ester hydrolase activity; 5'-3' exonuclease activity; DNA binding; manganese ion binding; endonuclease activity; exonuclease activity; ribonuclease H activity; catalytic activity

Biological Process: DNA replication, removal of RNA primer; DNA replication; DNA repair; response to DNA damage stimulus; DNA catabolic process, endonucleolytic; memory