Mouse FELINE LEUKAEMIA VIRUS p27 Monoclonal Antibody | anti-gag-pro-pol antibody
MOUSE ANTI FELINE LEUKAEMIA VIRUS p27
Applications
ELISA, Immunohistochemistry, Western Blot
Synonyms
FELINE LEUKAEMIA VIRUS p27; Monoclonal Antibody; MOUSE ANTI FELINE LEUKAEMIA VIRUS p27; anti-gag-pro-pol antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
PF12J-10A
Specificity
This item recognises feline leukaemia virus (FeLV) p27 core protein (p27).
The oncoretrovirus FeLV is responsible for both neoplastic and non-neoplastic conditions, often resulting in a poor prognosis and fatal outcome for cats persistently infected. During persistent infection the virus, soluble viral antigens and antigen containing white blood cells are continuously present in the blood is mainly based upon detection of the p27 antigen - using ELISA to test for p27 as it circulates free in the bloodstream, and Immunofluorescence studies to detect p27 within the infected white cells.
The oncoretrovirus FeLV is responsible for both neoplastic and non-neoplastic conditions, often resulting in a poor prognosis and fatal outcome for cats persistently infected. During persistent infection the virus, soluble viral antigens and antigen containing white blood cells are continuously present in the blood is mainly based upon detection of the p27 antigen - using ELISA to test for p27 as it circulates free in the bloodstream, and Immunofluorescence studies to detect p27 within the infected white cells.
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Sequence Length
1786
Applicable Applications for anti-gag-pro-pol antibody
ELISA (EIA), Immunohistology Paraffin, Western Blot (WB)
Application Notes
Western Blot: This item detects a band of approximately 28kDa under reducing conditions. A strong band of 80kDa representing glycosylated FeLV gag gene product may also be present.
Immunohistology - Paraffin: Maximum Dilution: 1/100
Western Blotting: Maximum Dilution: 1/1,500
Immunohistology - Paraffin: Maximum Dilution: 1/100
Western Blotting: Maximum Dilution: 1/1,500
Perservative Stabilisers
0.05% Sodium Azide (NaN3)
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Target Species
Viral
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Related Product Information for anti-gag-pro-pol antibody
MBS212873 recognises feline leukaemia virus (FeLV) p27 core protein (p27). The oncoretrovirus FeLV is responsible for both neoplastic and non-neoplastic conditions, often resulting in a poor prognosis and fatal outcome for cats persistently infected. During persistent infection the virus, soluble viral antigens and antigen containing white blood cells are continuously present in the blood and identification is mainly based upon detection of the p27 antigen - using ELISA to test for p27 as it circulates free in the bloodstream, and Immunofluorescence studies to detect p27 within the infected white cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
200,202 Da
NCBI Official Full Name
Gag-Pro-Pol polyprotein gPr80
NCBI Official Symbol
gag-pro-pol
NCBI Protein Information
Gag-Pro-Pol precursor polyprotein gPr80; Matrix; virion core structural peptide p12; Capsid; Nucleocapsid; Gag polyprotein Pr65-gag; Pol gene polyprotein; Protease (PR); Reverse transcriptase (RT); Endonuclease/integrase (IN)
UniProt Protein Name
Gag-pol polyprotein gPr80
Protein Family
UniProt Entry Name
O89811_FLV
Similar Products
Product Notes
The gag-pro-pol (Catalog #AAA212873) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's FELINE LEUKAEMIA VIRUS p27 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistology Paraffin, Western Blot (WB). Western Blot: This item detects a band of approximately 28kDa under reducing conditions. A strong band of 80kDa representing glycosylated FeLV gag gene product may also be present. Immunohistology - Paraffin: Maximum Dilution: 1/100 Western Blotting: Maximum Dilution: 1/1,500. Researchers should empirically determine the suitability of the gag-pro-pol for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FELINE LEUKAEMIA VIRUS p27, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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