Mouse FceRI, alpha Monoclonal Antibody | anti-FCER1A antibody

FceRI, alpha (Fc epsilon RI, IgE Receptor)

Cat. Num. AAA632728

• Gene Names: FCER1A; FCE1A; FcERI
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: FceRI; alpha; Monoclonal Antibody; alpha (Fc epsilon RI; IgE Receptor); Anti -FceRI; anti-FCER1A antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 0.T.195
• Specificity: Recognizes rat FceRI alpha at ~45-55kD.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in 0.1M Tris-glycine, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
• Concentration: 1 mg/mL (varies by lot)

• Applicable Applications for anti-FCER1A antibody: Suitable for use in Immunoprecipitation/Western Blot and FACS. Not suitable for Western Blot without pre-immunoprecipitation of antibody. ; ; Other applications have not been tested.
• Application Notes: Immunoprecipitation: 5 ug immunoprecipitates rat FceRI alpha from 500 ug of biotinylated rat basophilic leukemia cell lysate. The immunoprecipitate was resolved by electrophoresis and transferred to nitrocellulose. Biotinylated protein bands wew visualized using NeutrAvidin labeled with HRP and and enhanced chemiluminesce detection system.; ; Optimal dilutions to be determined by the researcher.
• Immunogen: Whole rat basophilic leukemia cells, positive for the rat high-affinity receptor recognizing immunoglobulin E (FceRI).
• Preparation and Storage: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(IP/WB: Biotinylated rat basophilic leukemic cell lysate was incubated with MBS632728 (5ug).)

Related Product Information for anti-FCER1A antibody

The allergic reaction is mediated through the IgE high affinity receptor (FceRI). When a multivalent antigen is presented, a redistribution of the monomeric IgE-FceRI receptor complexes cause the degranulation of mast cells and basophils, resulting in the subsequent release of factors responsible for the allergic reaction. The FceRI receptor is a tetrameric complex, consisting of an a-chain, a b-chain and a dimeric g-chain. While the a-chain is primarily involved with the binding of IgE, the signal is transferred through the g subunit, which contains the immunoreceptor tyrosine activation motifs (ITAM) critical for initiating receptor mediated signal transduction through the Syk and Lyn tyrosine kinases. The FceRI receptor is able to engage and disengage the IgE, providing a versatile model for studying the function of kinase coupled receptors.

Product Categories/Family for anti-FCER1A antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 15929530
• NCBI GeneID: 2205
• UniProt Accession #: P12319
• NCBI Official Full Name: Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
• NCBI Official Synonym Full Names: Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
• NCBI Official Symbol: FCER1A
• NCBI Official Synonym Symbols: FCE1A; FcERI
• NCBI Protein Information: high affinity immunoglobulin epsilon receptor subunit alpha; Fc-epsilon RI-alpha; Fc epsilon RI alpha-chain; igE Fc receptor subunit alpha; Fc IgE receptor, alpha polypeptide; high affinity immunoglobulin epsilon receptor alpha-subunit; immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide
• UniProt Protein Name: High affinity immunoglobulin epsilon receptor subunit alpha
• Protein Family: High affinity immunoglobulin epsilon receptor
• UniProt Gene Name: FCER1A
• UniProt Synonym Gene Names: FCE1A; FcERI
• UniProt Entry Name: FCERA_HUMAN

NCBI Description

The immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine that are responsible for allergy symptoms are released. This receptor is comprised of an alpha subunit, a beta subunit, and two gamma subunits. The protein encoded by this gene represents the alpha subunit. [provided by RefSeq, Aug 2011]

Uniprot Description

Function: Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.

Subunit structure: Tetramer of an alpha chain, a beta chain, and two disulfide linked gamma chains.

Subcellular location: Cell membrane; Single-pass type I membrane protein.

Sequence similarities: Contains 2 Ig-like (immunoglobulin-like) domains.