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Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant FBXO21 protein (Right) or empty vector (Left) detected with FBXO21 antibody)

Mouse FBXO21 Monoclonal Antibody | anti-FBXO21 antibody

FBXO21 antibody

Gene Names
FBXO21; FBX21
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
FBXO21 antibody was purified by affinity chromatography.
Synonyms
FBXO21; Monoclonal Antibody; FBXO21 antibody; Monoclonal FBXO21; Anti-FBXO21; F-Box Protein 21; DKFZp434G058; FBX21; FLJ90233; KIAA0875; MGC26682; FBXO 21; FBXO-21; anti-FBXO21 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6C10
Purity/Purification
FBXO21 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
628
Applicable Applications for anti-FBXO21 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC: 1:100
IHC: 1:150
WB: 1:2000
Biological Significance
FBXO21 contains 1 F-box domain. It is a substrate-recognition component of the SCF (SKP1-Cul1-F-box protein)-type E3 ubiquitin ligase complex.
Cross-Reactivity
Human
Immunogen
FBXO21 antibody was raised in mouse using a full length recombinant protein of human FBXO21 (NP_296373) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant FBXO21 protein (Right) or empty vector (Left) detected with FBXO21 antibody)

Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant FBXO21 protein (Right) or empty vector (Left) detected with FBXO21 antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of FBXO21 protein in paraffin embedded Human ovary tissue using FBXO21 antibody)

Immunohistochemistry (IHC) (Immunohistochemical analysis of FBXO21 protein in paraffin embedded Human ovary tissue using FBXO21 antibody)
Related Product Information for anti-FBXO21 antibody
Mouse monoclonal FBXO21 antibody
Product Categories/Family for anti-FBXO21 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
71,479 Da
NCBI Official Full Name
F-box only protein 21 isoform 1
NCBI Official Synonym Full Names
F-box protein 21
NCBI Official Symbol
FBXO21
NCBI Official Synonym Symbols
FBX21
NCBI Protein Information
F-box only protein 21
UniProt Protein Name
F-box only protein 21
Protein Family
UniProt Gene Name
FBXO21
UniProt Synonym Gene Names
FBX21; KIAA0875
UniProt Entry Name
FBX21_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

FBXO21: Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 12q24.22

Cellular Component: ubiquitin ligase complex

Molecular Function: DNA binding; ubiquitin-protein ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; protein ubiquitination

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Product Notes

The FBXO21 fbxo21 (Catalog #AAA532815) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's FBXO21 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB). FC: 1:100 IHC: 1:150 WB: 1:2000. Researchers should empirically determine the suitability of the FBXO21 fbxo21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FBXO21, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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