Mouse anti-Human FANCJ Monoclonal Antibody | anti-BRIP1 antibody

FANCJ (FANCJ protein, BACH 1, BACH1 BRIP1, BRIP 1, BRAC 1 Associated C Terminal Helicase 1, BRCA 1 Interacting Protein 1)

Cat. Num. AAA602035

• Gene Names: BRIP1; OF; BACH1; FANCJ; FLJ90232; MGC126521; MGC126523
• Reactivity: Human
• Applications: Western Blot
• Purity: Ascites
• Synonyms: FANCJ; Monoclonal Antibody; FANCJ (FANCJ protein; BACH 1; BACH1 BRIP1; BRIP 1; BRAC 1 Associated C Terminal Helicase 1; BRCA 1 Interacting Protein 1); Anti -FANCJ (FANCJ protein; anti-BRIP1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 8k153
• Specificity: Specific for human Brip1.
• Purity/Purification: Ascites

• Applicable Applications for anti-BRIP1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Fusion protein 647-1043 of human Brip1.
• Positive Control: HeLa whole and nuclear extracts, Raji whole cell extracts.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-BRIP1 antibody

BRIP1 (also called BACH1) is a helicase that interacts with the BRCT domain of BRCA1 and has a role in BRCA1-dependent DNA repair and checkpoint. BRIP1 has recently been found to be defective in Fanconi anemia complementation group J. BRIP1/FANCJ has a function in the Fanconi anemia pathway that is independent of BRCA1 and downstream of FANCD2 activation. (1-4)

Product Categories/Family for anti-BRIP1 antibody

Antibodies; Abs to DNA Replication; Repair

NCBI and Uniprot Product Information

• NCBI GI #: 14042978
• NCBI GeneID: 83990
• NCBI Accession #: NP_114432.1
• NCBI GenBank Nucleotide #: NM_032043.1
• UniProt Accession #: Q9BX63; Q3MJE2; Q8NCI5
• Molecular Weight: 140,878 Da
• NCBI Official Full Name: Fanconi anemia group J protein
• NCBI Official Synonym Full Names: BRCA1 interacting protein C-terminal helicase 1
• NCBI Official Symbol: BRIP1
• NCBI Official Synonym Symbols: OF; BACH1; FANCJ; FLJ90232; MGC126521; MGC126523
• NCBI Protein Information: Fanconi anemia group J protein; ATP-dependent RNA helicase BRIP1; BRCA1/BRCA2-associated helicase 1; BRCA1-associated C-terminal helicase 1; BRCA1-binding helicase-like protein BACH1
• UniProt Protein Name: Fanconi anemia group J protein
• Protein Family: Fanconi anemia group J protein
• UniProt Gene Name: BRIP1
• UniProt Synonym Gene Names: BACH1; FANCJ
• UniProt Entry Name: FANCJ_HUMAN

NCBI Description

The protein encoded by this gene is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). This gene may be a target of germline cancer-inducing mutations. [provided by RefSeq]

Uniprot Description

BRIP1: a DNA-dependent ATPase and DNA helicase required for the maintenance of chromosomal stability. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1. Binds directly to the BRCT domains of BRCA1. Defects in BRIP1 cause of susceptibility to breast cancer and Fanconi anemia. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Belongs to the DEAD box helicase family, DEAH subfamily. Two alternatively spliced human isoforms have been described.

Protein type: EC 3.6.4.13; Oncoprotein; Helicase

Chromosomal Location of Human Ortholog: 17q22.2

Cellular Component: nuclear membrane; cytoplasm; nucleus

Molecular Function: ATP-dependent DNA helicase activity; protein binding; DNA binding; metal ion binding; 4 iron, 4 sulfur cluster binding; ATP binding

Biological Process: regulation of transcription from RNA polymerase II promoter; double-strand break repair; DNA damage checkpoint; DNA duplex unwinding

Disease: Fanconi Anemia, Complementation Group J; Breast Cancer; Tracheoesophageal Fistula With Or Without Esophageal Atresia