Mouse anti-Human Factor XII Light Chain Monoclonal Antibody
Factor XII Light Chain (Hageman Factor)
Reactivity
Human
Applications
ELISA, Neutralization Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Factor XII Light Chain; Monoclonal Antibody; Factor XII Light Chain (Hageman Factor); Anti -Factor XII Light Chain (Hageman Factor); anti-Factor XII Light Chain antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1.B.694
Specificity
Recognizes the light chain of human Factor XII and at 100nM inhibits 50% of Factor XII activity.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4. No preservative added.
Applicable Applications for anti-Factor XII Light Chain antibody
ELISA (EL/EIA), Neutralization
Application Notes
Suitable for use in ELISA, Inhibition and Coagulation assays.
Immunogen
Purified human Factor XII.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Factor XII Light Chain antibody
Human Factor XII (XII) (Hageman Factor) is a single chain (78kD) glycoprotein zymogen that circulates in plasma at a concentration of 40 mg/ml. Reciprical activation of XII to the active serine protease factor XIIa (XIIa) by kallikrein is central to initiation of the intrinsic coagulation pathway. Surface bound a-XIIa in turn activates factor XI to XIa. A single cleavage by kallikrein at R353-Val354 of XII yields a-XIIa, a 2 chain protease (80kD) held together by disulfide bonds. The COOH-terminal light chain (28kD) contains the catalytic triad (His-40, Asp-89, Ser-191), while the NH2-terminal heavy chain (52kD) conatins the anionic surface binding portion of the molecule. A secondary cleavage of a-XIIa by kallikrein outside the disulfide bond yields b-XIIa (XIIf, BHFa, HFf, hageman factor fragments) (28kD), which no longer binds anionic surfaces. b-XIIa can activate prekallikrein, but has little procoagulant activity.
Product Categories/Family for anti-Factor XII Light Chain antibody
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Product Notes
The Factor XII Light Chain (Catalog #AAA602874) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Factor XII Light Chain (Hageman Factor) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Factor XII Light Chain can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Neutralization. Suitable for use in ELISA, Inhibition and Coagulation assays. Researchers should empirically determine the suitability of the Factor XII Light Chain for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Factor XII Light Chain, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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