Mouse anti-Human Factor VIII Monoclonal Antibody

Factor VIII (MaxLight 550)

Cat. Num. AAA6209618

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: Factor VIII; Monoclonal Antibody; Factor VIII (MaxLight 550); anti-Factor VIII antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 9L234
• Specificity: Recognizes full-length human Factor VIII. Does not cross-react with von Willebrand factor.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.4. Labeled with MaxLight550.

• Applicable Applications for anti-Factor VIII antibody: FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Purified Human Factor VIII.
• Conjugate: MaxLight550
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-Factor VIII antibody

Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. It is an extracellular factor. Defects in F8 are the cause of hemophilia A (HEMA). HEMA is a common recessive X linked coagulation disorder. The frequency of hemophilia A is 1-2 in 10,000 male births in all ethnic groups. About 50% of patients have severe hemophilia A with F8C activity less than 1% of normal; they have frequent spontaneous bleeding into joints, muscles and internal organs. Moderately severe hemophilia A occurs in about 10% of patients; F8C activity is 2-5% of normal, and there is bleeding after minor trauma. Mild hemophilia A, which occurs in 30-40% of patients, is associated with F8C activity of 5-30% and bleeding occurs only after significant trauma or surgery. Of particular interest for the understanding of the function of F8C is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8C in their plasma (at least 30% of normal), but the protein is nonfunctional; i.e., the F8C activity is much less than the plasma protein level. CRM reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein.

Product Categories/Family for anti-Factor VIII antibody

Antibodies; Coagulation Factors

References

1. Fay, et al., (1986), Biochim. Biophys. Acta., 87, 368-278, 2. Fay, (1988), Arch. Biochem. Biophys., 262, 525-531, 3. Fay, et al., (2001), J. Biol. Chem., 276, 12434-12439.