Mouse anti-Bovine Factor V, activation peptide, 120kD Monoclonal Antibody | anti-F5 antibody

Factor V, activation peptide, 120kD

Cat. Num. AAA606434

• Gene Names: F5; FVL; PCCF
• Reactivity: Bovine
• Applications: ELISA, Western Blot, immunoassay, Radioimmunoassay
• Purity: Purified; Purified (95% by SDS-PAGE)
• Synonyms: Factor V; activation peptide; 120kD; Monoclonal Antibody; Anti -Factor V; anti-F5 antibody

• Host: Mouse
• Reactivity: Bovine
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3H370
• Specificity: Recognizes the 120kD activation peptide of bovine Factor V. Does not react with murine factor Va via Western Blot.
• Purity/Purification: Purified; Purified (95% by SDS-PAGE)
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 50% glycerol.

• Applicable Applications for anti-F5 antibody: ELISA (EL/EIA), Western Blot (WB), Radioimmunoassay (RIA)
• Application Notes: Suitable for use in RIA, ELISA and Western Blot.; Dilution: Western Blot: 5ug/ml
• Immunogen: Purified Bovine Factor V
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-F5 antibody

Factor V is a large, single chain, plasma glycoprotein which is an essential component in the blood coagulation cascade. During coagulation, the procofactor, factor V, is converted to the active cofactor, factor Va, via limited proteolysis by the serine protease a-thrombin, and less efficiently by factor Xa. The active cofactor is composed of an NH2-terminal derived heavy chain (Mr=94,000) and a COOH-terminal derived light chain (Mr=74,000) which remain non-covalently associated in the presence of calcium ions. Factor Va serves as a cofactor for the serine protease factor Xa. Factor Va and Xa assemble on a phospholipid surface in a non-covalent and calcium ion-dependent manner, to form the prothrombinase complex. The prothrombinase complex is responsible for the rapid conversion of the zymogen prothrombin to the active serine protease, a-thrombin. Assembly of the prothrombinase complex increases the rate at which prothrombin is converted to thrombin by nearly 300,000-fold relative to the rate with factor Xa alone. Down regulation of the prothrombinase complex is accomplished partly through the inactivation of factor Va by activated protein C.

Product Categories/Family for anti-F5 antibody

Antibodies; Abs to Coagulation Factors

NCBI and Uniprot Product Information

• NCBI GI #: 182798
• NCBI GeneID: 2153
• UniProt Accession #: P12259; Q14285; Q2EHR5; Q5R346; Q5R347; Q6UPU6; Q8WWQ6; A8K6E8; Q15430; Q1L610; Q2HZY9; Q2HZZ0; Q2HZZ1; Q2I258; Q8IUL1; Q8TD21; Q8WWQ7
• Molecular Weight: 251,703 Da
• NCBI Official Full Name: factor V precusor
• NCBI Official Synonym Full Names: coagulation factor V (proaccelerin, labile factor)
• NCBI Official Symbol: F5
• NCBI Official Synonym Symbols: FVL; PCCF
• NCBI Protein Information: coagulation factor V; factor V Leiden; OTTHUMP00000032547; OTTHUMP00000032548; proaccelerin, labile factor; activated protein c cofactor; coagulation factor V jinjiang A2 domain
• UniProt Protein Name: Coagulation factor V
• Protein Family: Coagulation factor
• UniProt Gene Name: F5
• UniProt Entry Name: FA5_HUMAN

NCBI Description

This gene encodes an essential cofactor of the blood coagulation cascade. This factor circulates in plasma, and is converted to the active form by the release of the activation peptide by thrombin during coagulation. This generates a heavy chain and a light chain which are held together by calcium ions. The activated protein is a cofactor that participates with activated coagulation factor X to activate prothrombin to thrombin. Defects in this gene result in either an autosomal recessive hemorrhagic diathesis or an autosomal dominant form of thrombophilia, which is known as activated protein C resistance. [provided by RefSeq]

Uniprot Description

factor V: Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin. Defects in F5 are the cause of factor V deficiency (FA5D); also known as Owren parahemophilia. It is an hemorrhagic diastesis. Defects in F5 are the cause of thrombophilia due to activated protein C resistance (THPH2). THPH2 is a hemostatic disorder due to defective degradation of factor Va by activated protein C. It is characterized by a poor anticoagulant response to activated protein C resulting in tendency to thrombosis. Defects in F5 are a cause of susceptibility to Budd- Chiari syndrome (BDCHS). A syndrome caused by obstruction of hepatic venous outflow involving either the hepatic veins or the terminal segment of the inferior vena cava. Obstructions are generally caused by thrombosis and lead to hepatic congestion and ischemic necrosis. Clinical manifestations observed in the majority of patients include hepatomegaly, right upper quadrant pain and abdominal ascites. Budd-Chiari syndrome is associated with a combination of disease states including primary myeloproliferative syndromes and thrombophilia due to factor V Leiden, protein C deficiency and antithrombin III deficiency. Budd-Chiari syndrome is a rare but typical complication in patients with polycythemia vera. Defects in F5 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in F5 are associated with susceptibility to pregnancy loss, recurrent, type 1 (RPRGL1). RPRGL1 is a common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions. Belongs to the multicopper oxidase family.

Protein type: Secreted, signal peptide; Protease; Secreted

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: Golgi apparatus; extracellular space; membrane; endoplasmic reticulum; extracellular region; plasma membrane; vesicle

Molecular Function: protein binding; copper ion binding; serine-type endopeptidase activity

Biological Process: platelet activation; platelet degranulation; blood circulation; proteolysis; blood coagulation

Disease: Thrombophilia Due To Activated Protein C Resistance; Budd-chiari Syndrome; Stroke, Ischemic; Pregnancy Loss, Recurrent, Susceptibility To, 1; Factor V Deficiency