Western Blot (WB)
(Western Blot Positive WB detected in: MCF-7 whole cell lysate, THP-1 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Mouse Liver whole cell lysate All lanes: FAAH1 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 64 kDa Observed band size: 64 kDa)
FAAH recombinant antibody
FAAH Antibody
IHC: 1:50-1:200
Western Blot (WB)
(Western Blot Positive WB detected in: MCF-7 whole cell lysate, THP-1 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Mouse Liver whole cell lysate All lanes: FAAH1 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 64 kDa Observed band size: 64 kDa)
Western Blot (WB)
(Western Blot Positive WB detected in: MCF-7 whole cell lysate, THP-1 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Mouse Liver whole cell lysate All lanes: FAAH1 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 64 kDa Observed band size: 64 kDa)
Immunohistochemistry (IHC)
(IHC image diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
Immunohistochemistry (IHC)
(IHC image diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that is responsible for the hydrolysis of a number of primary and secondary fatty acid amides, including the neuromodulatory compounds anandamide and oleamide. [provided by RefSeq, Jul 2008]
Uniprot Description
FAAH: Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates. Homodimer. Highly expressed in the brain, small intestine, pancreas, skeletal muscle and testis. Also expressed in the kidney, liver, lung, placenta and prostate. Inhibited by O-aryl carbamates and alpha-keto heterocytes. Belongs to the amidase family.
Protein type: EC 3.5.1.99; Membrane protein, integral; Hydrolase
Chromosomal Location of Human Ortholog: 1p35-p34
Cellular Component: cytoskeleton; cytoplasm; integral to membrane; endomembrane system
Molecular Function: fatty acid amide hydrolase activity; carbon-nitrogen ligase activity, with glutamine as amido-N-donor; acylglycerol lipase activity
Biological Process: fatty acid catabolic process
Disease: Polysubstance Abuse, Susceptibility To
Research Articles on FAAH
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Product Notes
The FAAH faah (Catalog #AAA7136681) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FAAH Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's FAAH can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:5000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the FAAH faah for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAAH, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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