Immunofluorescence (IF)
(Fluorescent image of U251 cell stained with EZH2 Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EZH2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).EZH2 immunoreactivity is localized to Nucleus significantly.)
Mouse EZH2 Monoclonal Antibody | anti-EZH2 antibody
EZH2 Antibody
WB~~1:100~500
IHC~~1:10~50
Immunofluorescence (IF)
(Fluorescent image of U251 cell stained with EZH2 Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EZH2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).EZH2 immunoreactivity is localized to Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent image of U251 cell stained with EZH2 Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EZH2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).EZH2 immunoreactivity is localized to Nucleus significantly.)
Western Blot (WB)
(Western blot analysis of EZH2 antibody in T47D cell line lysates (35ug/lane).EZH2 (arrow) was detected using the purified Mab.)
Western Blot (WB)
(Western blot analysis of EZH2 antibody in T47D cell line lysates (35ug/lane).EZH2 (arrow) was detected using the purified Mab.)
Immunohistochemistry (IHC)
(EZH2 Monoclonal Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EZH2 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(EZH2 Monoclonal Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EZH2 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Feb 2011]
Uniprot Description
EZH2: a histone H3 Lys 27 (H3K27) methyltransferase and Polycomb group protein with oncogenic activity. A master regulatory protein that plays a critical role in development. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates K9 and K27 of histone H3, leading to transcriptional repression of the affected target genes. PRC2 includes the Ezh2, EED, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The recruitment of PRC2 to a promoter leads to increased levels of di- and trimethylation of H3K27 (H3K27me2/3), regulating the balance between self-renewal and differentiation of embryonic stem cells (ESCs). The recruitment of PRC2 to promoters in ES cells is interdependent on Jarid 2. ESCs lacking the PRC2 component EED are deficient in Jarid2 promoter activity, while the knockdown of Jarid 2 reduces PRC2 at its target promoters. Able to mono-, di- and trimethylate K27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, p14ARF and retinoic acid target genes. Binds ATRX via the SET domain. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with HDAC1 and HDAC2. Interacts with PRAME. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis. Expression decreases during senescence of embryonic fibroblasts. Expression peaks at the G1/S phase boundary. Expression is induced by E2F1, E2F2 and E2F3. Expression is reduced in cells subject to numerous types of stress including UV-, IR- and bleomycin-induced DNA damage and by activation of p53. It has been reported that phosphorylation by AKT1 on S21 reduces methyltransferase activity.
Protein type: EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine
Chromosomal Location of Human Ortholog: 7q35-q36
Cellular Component: nucleoplasm; pronucleus; ESC/E(Z) complex; cytoplasm; nuclear chromatin; nucleus
Molecular Function: histone methyltransferase activity; protein binding; DNA binding; sequence-specific DNA binding; RNA binding; chromatin DNA binding; protein-lysine N-methyltransferase activity; chromatin binding; histone-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K27 specific)
Biological Process: negative regulation of striated muscle cell differentiation; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of gliogenesis; rhythmic process; negative regulation of transcription from RNA polymerase II promoter; satellite cell compartment self-renewal involved in skeletal muscle regeneration; regulation of circadian rhythm; regulation of gene expression, epigenetic; regulation of cell proliferation; positive regulation of MAP kinase activity; negative regulation of retinoic acid receptor signaling pathway; cerebellar cortex development; regulation of transcription, DNA-dependent; negative regulation of gene expression, epigenetic; negative regulation of epidermal cell differentiation; DNA methylation; gene expression; negative regulation of transcription, DNA-dependent
Disease: Weaver Syndrome
Research Articles on EZH2
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Product Notes
The EZH2 ezh2 (Catalog #AAA9200515) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EZH2 Antibody reacts with Human (Predicted Reactivity: Monkey) and may cross-react with other species as described in the data sheet. AAA Biotech's EZH2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF). IF~~1:10~50 WB~~1:100~500 IHC~~1:10~50. Researchers should empirically determine the suitability of the EZH2 ezh2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EZH2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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