Western Blot (WB)
(Fig. Western blot analysis of EYFP fusion protein with anti-EYFP tag monoclonal Antibody (10T3) at 1:10000 (lane A) dilution.)
Mouse EYFP-Tag Monoclonal Antibody | anti-EYFP antibody
Anti-EYFP-Tag Monoclonal Antibody (10T3)
Applications
Western Blot, Immunoprecipitation
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Synonyms
EYFP-Tag; Monoclonal Antibody; Anti-EYFP-Tag Monoclonal Antibody (10T3); eYFP; YFP; Yellow Fluorescent Protein; enhanced Yellow Fluorescent Protein; anti-EYFP antibody
Host
Mouse
Clonality
Monoclonal
Isotype
Mouse IgG
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Form/Format
Liquid in PBS, pH7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Applicable Applications for anti-EYFP antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:5000
Tag
EYFP
Immunogen
Recombinant Protein
Preparation and Storage
Stable for one year at -20 degree C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping||Gel pack with blue ice.
Shipping||Gel pack with blue ice.
Western Blot (WB)
(Fig. Western blot analysis of EYFP fusion protein with anti-EYFP tag monoclonal Antibody (10T3) at 1:10000 (lane A) dilution.)
Western Blot (WB)
(Fig. Western blot analysis of EYFP fusion protein with anti-EYFP tag monoclonal Antibody (10T3) at 1:10000 (lane A) dilution.)
Related Product Information for anti-EYFP antibody
Description: Featured Anti-EYFP-Tag Polyclonal Antibody (10T3), specially designed for your taged protein quantification and localization.
Background: Enhanced Yellow florescent protein.Yellow Fluorescent Protein (YFP) is a genetic mutant of green fluorescent protein, derived from Aequorea victoria. Its excitation peak is 514nm and its emission peak is 527nm. Like green fluorescent protein (GFP), it is a useful tool in cell and molecular biology, usually explored using fluorescence microscopy. Three improved versions of YFP are Citrine, Venus, and Ypet. They have reduced chloride sensitivity, faster maturation, and increased brightness (product of the extinction coefficient and quantum yield). Typically, yellow FPs serve as the acceptor for genetically-encoded FRET sensors of which the most likely donor FP is mCFP (monomeric cyan FP). The red-shift relative to GFP is caused by a Pi-Pi stacking interaction as a result of the T203Y mutation, which essentially increases the polarizability of the local chromophore environment as well as providing additional electron density into the chromophore.
Background: Enhanced Yellow florescent protein.Yellow Fluorescent Protein (YFP) is a genetic mutant of green fluorescent protein, derived from Aequorea victoria. Its excitation peak is 514nm and its emission peak is 527nm. Like green fluorescent protein (GFP), it is a useful tool in cell and molecular biology, usually explored using fluorescence microscopy. Three improved versions of YFP are Citrine, Venus, and Ypet. They have reduced chloride sensitivity, faster maturation, and increased brightness (product of the extinction coefficient and quantum yield). Typically, yellow FPs serve as the acceptor for genetically-encoded FRET sensors of which the most likely donor FP is mCFP (monomeric cyan FP). The red-shift relative to GFP is caused by a Pi-Pi stacking interaction as a result of the T203Y mutation, which essentially increases the polarizability of the local chromophore environment as well as providing additional electron density into the chromophore.
Product Categories/Family for anti-EYFP antibody
Similar Products
Product Notes
The EYFP (Catalog #AAA9719166) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's EYFP-Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:5000. Researchers should empirically determine the suitability of the EYFP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EYFP-Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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