Testing Data
(Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor alpha antibody (MBS214054) followed by HRP polymer detection and DAB substrate develoment following heat mediated antigen retrieval using citrate buffer at pH 6.2 (low power))
Mouse ESTROGEN RECEPTOR ALPHA Monoclonal Antibody | anti-ESR1 antibody
MOUSE ANTI HUMAN ESTROGEN RECEPTOR ALPHA
Immunohistology - Paraffin: Minimum Dilution: 1/40; Maximum Dilution: 1/80; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Histology Positive Control Tissue
Shelf Life: 18 months from date of despatch.
Testing Data
(Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor alpha antibody (MBS214054) followed by HRP polymer detection and DAB substrate develoment following heat mediated antigen retrieval using citrate buffer at pH 6.2 (low power))
Testing Data
(Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor alpha antibody (MBS214054) followed by HRP polymer detection and DAB substrate develoment following heat mediated antigen retrieval using citrate buffer at pH 6.2 (low power))
Testing Data
(Western blot analysis of MCF-7 human breast adenocarcinoma whole cell lysate probed with Mouse anti Human estrogen receptor alpha antibody (MBS214054) followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence)
Testing Data
(Western blot analysis of MCF-7 human breast adenocarcinoma whole cell lysate probed with Mouse anti Human estrogen receptor alpha antibody (MBS214054) followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence)
Testing Data
(Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor alpha antibody (MBS214054) followed by HRP polymer detection and DAB substrate develoment following heat mediated antigen retrieval using citrate buffer at pH 6.2 (high power))
Testing Data
(Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor alpha antibody (MBS214054) followed by HRP polymer detection and DAB substrate develoment following heat mediated antigen retrieval using citrate buffer at pH 6.2 (high power))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]
Uniprot Description
ER-alpha: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.
Protein type: DNA-binding; Nuclear receptor
Chromosomal Location of Human Ortholog: 6q25.1
Cellular Component: nucleoplasm; Golgi apparatus; membrane; cytoplasm; plasma membrane; integral to membrane; nucleus
Molecular Function: identical protein binding; estrogen receptor activity; zinc ion binding; nitric-oxide synthase regulator activity; beta-catenin binding; estrogen response element binding; transcription factor binding; steroid binding; protein binding; enzyme binding; steroid hormone receptor activity; chromatin binding; transcription factor activity
Biological Process: positive regulation of nitric oxide biosynthetic process; estrogen receptor signaling pathway; uterus development; signal transduction; response to estradiol stimulus; regulation of apoptosis; positive regulation of fibroblast proliferation; elevation of cytosolic calcium ion concentration; regulation of transcription, DNA-dependent; epithelial cell development; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; positive regulation of nitric-oxide synthase activity; antral ovarian follicle growth; negative regulation of transcription factor activity; male gonad development; androgen metabolic process; vagina development; positive regulation of retinoic acid receptor signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; chromatin remodeling; response to estrogen stimulus; positive regulation of transcription from RNA polymerase II promoter; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); gene expression; positive regulation of transcription factor activity; steroid hormone mediated signaling
Disease: Migraine With Or Without Aura, Susceptibility To, 1; Breast Cancer; Myocardial Infarction, Susceptibility To; Estrogen Resistance
Research Articles on ESR1
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Product Notes
The ESR1 esr1 (Catalog #AAA215228) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ESTROGEN RECEPTOR ALPHA can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen*, Immunohistology Paraffin*. Immunohistology - Frozen: Minimum Dilution: 1/40; Maximum Dilution: 1/60; Application Note: The use of Zamboni\'s fixative is recommended for best results Immunohistology - Paraffin: Minimum Dilution: 1/40; Maximum Dilution: 1/80; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Researchers should empirically determine the suitability of the ESR1 esr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ESTROGEN RECEPTOR ALPHA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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