Immunofluorescence (IF)
(Immunofluorescence analysis of Hela cells using ESR1 antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Mouse ESR1 Monoclonal Antibody | anti-ESR1 antibody
ESR1 antibody
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Synonyms
ESR1; Monoclonal Antibody; ESR1 antibody; Mouse monoclonal ESR1 antibody; Monoclonal ESR1 antibody; Anti-ESR1 antibody; ESR 1; ESR-1; ESR 1 antibody; ESR-1 antibody; anti-ESR1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6B6
Form/Format
Liquid in ascitic fluid containing 0.03% sodium azide.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
229
Applicable Applications for anti-ESR1 antibody
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
ELISA: 1:10,000
IF: 1:200-1:1000
IHC: 1:200-1:1000
WB: 1:500-1:2000
IF: 1:200-1:1000
IHC: 1:200-1:1000
WB: 1:500-1:2000
Immunogen
ESR1 antibody was raised in Mouse using a purified recombinant fragment of human ESR1 expressed in E Coli as the immunogen.
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage.
Store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence analysis of Hela cells using ESR1 antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Immunofluorescence (IF)
(Immunofluorescence analysis of Hela cells using ESR1 antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Western Blot (WB)
(Western Blot showing ESR1 antibody used against MCF-7 (1), T47D (2) and SKBR3 (3) cell lysate.)
Western Blot (WB)
(Western Blot showing ESR1 antibody used against MCF-7 (1), T47D (2) and SKBR3 (3) cell lysate.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mammary cancer tissues using ESR1 antibody with DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mammary cancer tissues using ESR1 antibody with DAB staining.)
Related Product Information for anti-ESR1 antibody
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.
Product Categories/Family for anti-ESR1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25,913 Da
NCBI Official Full Name
ESR1, partial
NCBI Official Synonym Full Names
estrogen receptor 1
NCBI Official Symbol
ESR1
NCBI Protein Information
estrogen receptor
UniProt Protein Name
ESR1
Protein Family
UniProt Gene Name
ESR1
Similar Products
Product Notes
The ESR1 esr1 (Catalog #AAA5310903) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ESR1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). ELISA: 1:10,000 IF: 1:200-1:1000 IHC: 1:200-1:1000 WB: 1:500-1:2000. Researchers should empirically determine the suitability of the ESR1 esr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ESR1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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