Mouse anti-Human ERN1 / IRE1 Monoclonal Antibody | anti-ERN1 antibody

Mouse Monoclonal [clone 3D1] (IgG1,k) to Human ERN1 / IRE1

Cat. Num. AAA245895

• Gene Names: ERN1; IRE1; IRE1P; IRE1a; hIRE1p
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: ERN1 / IRE1; Monoclonal Antibody; Mouse Monoclonal [clone 3D1] (IgG1; k) to Human ERN1 / IRE1; Anti-ERN1 / IRE1 Antibody (clone 3D1) IHC-plus; ERN1; ER to nucleus signalling 1; Inositol-requiring enzyme 1; Inositol-requiring protein 1; Ire1-alpha; IRE1a; HIRE1p; IRE1; Inositol-requiring 1; IRE1P; Human ERN1; anti-ERN1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3D1
• Specificity: Human IRE1
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Concentration: 0.39 mg/ml (varies by lot)
• Sequence Length: 977

• Applicable Applications for anti-ERN1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB; Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: cell lysate and recombinant protein.
• Target Species: Human
• Immunogen Description: ERN1 (AAC25991, 401 a.a. ~ 500 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: ERN1 / IRE1 antibody was raised against eRN1 (AAC25991, 401 a.a. ~ 500 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-ERN1 / IRE1 antibody IHC of human prostate, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(ERN1 monoclonal antibody clone 3D1 Western blot of ERN1 expression in HeLa.)

ELISA (EIA)

(Detection limit for recombinant GST tagged ERN1 is approximately 0.3 ng/ml as a capture antibody.)

Related Product Information for anti-ERN1 antibody

The endoribonuclease IRE1/ERN1 encodes the ER-to-nucleus signaling 1 protein, a human homolog of the yeast Ire1 gene product. In S. cerevisiae, as well as in human, this ER transmembrane IRE serine/threonine protein kinase monitors the status of unfolded proteins inside the ER lumen in response to stress. Activation of ERN1 leads to oligomerization, autophosphorylation, and proteolytic cleavage.

Product Categories/Family for anti-ERN1 antibody

Family: Protein Kinase; Subfamily: IRE

NCBI and Uniprot Product Information

• NCBI GI #: 153946421
• NCBI GeneID: 2081
• NCBI Accession #: NP_001424.3
• NCBI GenBank Nucleotide #: NM_001433.3
• UniProt Accession #: O75460; Q59EE2; A1L457; A8K8N8; A8MXS7
• Molecular Weight: 6,649 Da
• NCBI Official Full Name: serine/threonine-protein kinase/endoribonuclease IRE1
• NCBI Official Synonym Full Names: endoplasmic reticulum to nucleus signaling 1
• NCBI Official Symbol: ERN1
• NCBI Official Synonym Symbols: IRE1; IRE1P; IRE1a; hIRE1p
• NCBI Protein Information: serine/threonine-protein kinase/endoribonuclease IRE1; ERN1; ire1-alpha; inositol-requiring 1; ER to nucleus signalling 1; inositol-requiring enzyme 1; inositol-requiring protein 1; endoplasmic reticulum-to-nucleus signaling 1
• UniProt Protein Name: Serine/threonine-protein kinase/endoribonuclease IRE1
• UniProt Gene Name: ERN1
• UniProt Synonym Gene Names: hIRE1p; IRE1a
• UniProt Entry Name: ERN1_HUMAN

NCBI Description

The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq, Jul 2008]

Uniprot Description

IRE1: a ser/thr protein kinase that possess endonuclease activity. Important in altering gene expression as a response to endoplasmic reticulum based stress signals. Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain, which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. The kinase domain is activated by trans-autophosphorylation. Kinase activity is required for activation of the endoribonuclease domain. Ubiquitously expressed. High levels observed in pancreatic tissue. Functionally connected with insulin biosynthesis in pancreatic beta cells. Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds HSPA5, a negative regulator of the unfolded protein response. This interaction may disrupt homodimerization and prevent activation of IRE1.

Protein type: EC 2.7.11.1; Protein kinase, Other; Endoplasmic reticulum; Kinase, protein; Membrane protein, integral; Chaperone; Protein kinase, Ser/Thr (non-receptor); Apoptosis; Ribonuclease; Other group; IRE family

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: endoplasmic reticulum membrane; mitochondrion; endoplasmic reticulum; cytoplasm; nuclear inner membrane; integral to endoplasmic reticulum membrane

Molecular Function: endoribonuclease activity; protein serine/threonine kinase activity; identical protein binding; protein binding; enzyme binding; protein homodimerization activity; magnesium ion binding; ADP binding; ATP binding

Biological Process: transcription, DNA-dependent; unfolded protein response; RNA splicing; protein amino acid autophosphorylation; protein amino acid phosphorylation; positive regulation of RNA splicing; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; regulation of transcription, DNA-dependent; endothelial cell proliferation; UFP-specific transcription factor mRNA processing during unfolded protein response; mRNA catabolic process; cell cycle arrest; regulation of macroautophagy; mRNA cleavage