Western Blot (WB)
(ERN1 monoclonal antibody (M01), clone 4D12 Western Blot analysis of ERN1 expression in HeLa (Cat # L013V1).)
Mouse ERN1 Monoclonal Antibody | anti-ERN1 antibody
ERN1 (Endoplasmic Reticulum to Nucleus Signaling 1, FLJ30999, IRE1, IRE1P, MGC163277, MGC163279) (Biotin)
Gene Names
ERN1; IRE1; IRE1P; IRE1a; hIRE1p
Applications
Western Blot
Purity
Purified
Synonyms
ERN1; Monoclonal Antibody; ERN1 (Endoplasmic Reticulum to Nucleus Signaling 1; FLJ30999; IRE1; IRE1P; MGC163277; MGC163279) (Biotin); Endoplasmic Reticulum to Nucleus Signaling 1; MGC163279; anti-ERN1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
4D12
Specificity
Recognizes ERN1.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Sequence Length
977
Applicable Applications for anti-ERN1 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
ERN1 (AAC25991, 401aa-500aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
EEVINLVDQTSENAPTTVSRDVEEKPAHAPARPEAPVDSMLKDMATIILSTFLLIGWVAFIITYPLSMHQQQQLQHQQFQKELEKIQLLQQQQQQLPFHP
Conjugate
Biotin
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(ERN1 monoclonal antibody (M01), clone 4D12 Western Blot analysis of ERN1 expression in HeLa (Cat # L013V1).)
Western Blot (WB)
(ERN1 monoclonal antibody (M01), clone 4D12 Western Blot analysis of ERN1 expression in HeLa (Cat # L013V1).)
Testing Data
(Detection limit for recombinant GST tagged ERN1 is approximately 0.3ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged ERN1 is approximately 0.3ng/ml as a capture antibody.)
Related Product Information for anti-ERN1 antibody
The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq]
Product Categories/Family for anti-ERN1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
protein kinase/endoribonulcease
NCBI Official Synonym Full Names
endoplasmic reticulum to nucleus signaling 1
NCBI Official Symbol
ERN1
NCBI Official Synonym Symbols
IRE1; IRE1P; IRE1a; hIRE1p
NCBI Protein Information
serine/threonine-protein kinase/endoribonuclease IRE1
NCBI Description
This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1. [provided by RefSeq, Aug 2017]
Research Articles on ERN1
Similar Products
Product Notes
The ERN1 (Catalog #AAA6172396) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ERN1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ERN1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERN1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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