Mouse ER-beta1 (Estrogen Receptor beta-1) Monoclonal Antibody | anti-ESR2 antibody

ER-beta1 (Estrogen Receptor beta-1) Mouse Monoclonal Antibody

Cat. Num. AAA438376

• Gene Names: ESR2; Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA
• Reactivity: Human, Monkey, Mouse, Rat, Pig, Horse, Sheep
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
• Synonyms: ER-beta1 (Estrogen Receptor beta-1); Monoclonal Antibody; ER-beta1 (Estrogen Receptor beta-1) Mouse Monoclonal Antibody; Erb; ESR BETA; ESR2; ESRB; ESTRB; estrogen nuclear receptor beta variant a; estrogen nuclear receptor beta variant b; estrogen receptor 2 (ER beta); estrogen receptor beta 4; anti-ESR2 antibody

• Host: Mouse
• Reactivity: Human, Monkey, Mouse, Rat, Pig, Horse, Sheep
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: ERb455
• Specificity: Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 495

• Applicable Applications for anti-ESR2 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); ; Optimal dilution for a specific application should be determined.
• Cellular Localization: Predominantly nuclear
• Immunogen: C-terminus fragment of recombinant human estrogen receptor beta protein
• Hu-Chromosome Location: 14q23.2
• Positive Control: MCF-7 Cells. Ovarian, Breast, Bladder, Gastric or Salivary carcinoma
• Preparation and Storage: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with ER-beta1 Monoclonal Antibody (ERb455).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with ER-beta1 Monoclonal Antibody (ERb455).)

Flow Cytometry (FC/FACS

(Flow Cytometry for human ER-beta on MCF-7 Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled ER-beta1 Monoclonal Antibody (ERb455).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with ER-beta1 Monoclonal Antibody (ERb455).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with ER-beta1 Monoclonal Antibody (ERb455).)

Flow Cytometry (FC/FACS

(Flow Cytometry of human ER beta on BT474 Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled ER beta1 Monoclonal Antibody (Erb455).)

SDS-PAGE (SDS)

(SDS-PAGE Analysis Purified Estrogen Receptor beta1 Mouse Monoclonal Antibody (ERb455). Confirmation of Integrity and Purity of Antibody.)

Western Blot (WB)

(Western Blot Analysis of human MCF-7 cell lysate using ER-beta1 Mouse Monoclonal Antibody (ERb455).)

References

Skliris GP et. al. J Pathol 2002;197:155-62.

NCBI and Uniprot Product Information

• NCBI GI #: 94538325
• NCBI GeneID: 2100
• NCBI Accession #: NP_001035365.1
• NCBI GenBank Nucleotide #: NM_001040275.1
• UniProt Accession #: Q92731; O60608; O60685; O60702; O60703; O75583; O75584; Q0MWT5; Q0MWT6; Q86Z31; A8K8K5; G3V5M5
• Molecular Weight: 53-59kDa
• NCBI Official Full Name: estrogen receptor beta isoform 2
• NCBI Official Synonym Full Names: estrogen receptor 2
• NCBI Official Symbol: ESR2
• NCBI Official Synonym Symbols: Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA
• NCBI Protein Information: estrogen receptor beta
• UniProt Protein Name: Estrogen receptor beta
• Protein Family: Estrogen receptor
• UniProt Gene Name: ESR2
• UniProt Synonym Gene Names: ESTRB; NR3A2; ER-beta
• UniProt Entry Name: ESR2_HUMAN

NCBI Description

This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

ER-beta: a nuclear hormone receptor and transcription factor. Binds and activated by estrogen. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Binds estrogens with an affinity similar to that of ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Eight alternatively-spliced isoforms have been described. Isoform beta-cx lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 14q23.2

Cellular Component: mitochondrion; nucleoplasm; nucleus

Molecular Function: DNA binding; enzyme binding; estrogen receptor activity; estrogen response element binding; protein binding; steroid binding; steroid hormone receptor activity; transcription coactivator activity; transcription factor activity

Biological Process: cell-cell signaling; estrogen receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent; signal transduction; transcription initiation from RNA polymerase II promoter