Western Blot (WB)
(Western Blot showing ER-alpha antibody used against human breast carcinoma tissue lysate.)
Mouse ER alpha Monoclonal Antibody | anti-ER alpha antibody
ER alpha antibody
Applications
ELISA, Immunohistochemistry, Western Blot
Synonyms
ER alpha; Monoclonal Antibody; ER alpha antibody; Monoclonal ER alpha; Anti-ER alpha; NR3A1; ERA; Estrogen receptor alpha; anti-ER alpha antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2B7
Form/Format
Purified antibody supplied in PBS containing 0.03% sodium azide.
Concentration
1.0 mg/ml (varies by lot)
Applicable Applications for anti-ER alpha antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
ELISA: 1:10,000
IHC: 1:200-1:1000
WB: 1:500-1:2000
IHC: 1:200-1:1000
WB: 1:500-1:2000
Biological Significance
The estrogen receptor (ESR) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Alternative splicing results in several ESR1 mRNA transcripts, which differ primarily in their 5-prime untranslated regions. The translated receptors show less variability.
Cross-Reactivity
Human
Immunogen
ER alpha antibody was raised in Mouse using a purified recombinant fragment of human ER-alpha (410-592aa) expressed in E Coli as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot showing ER-alpha antibody used against human breast carcinoma tissue lysate.)
Western Blot (WB)
(Western Blot showing ER-alpha antibody used against human breast carcinoma tissue lysate.)
Related Product Information for anti-ER alpha antibody
Mouse monoclonal ER alpha antibody
Product Categories/Family for anti-ER alpha antibody
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Product Notes
The ER alpha (Catalog #AAA830535) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ER alpha can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). ELISA: 1:10,000 IHC: 1:200-1:1000 WB: 1:500-1:2000. Researchers should empirically determine the suitability of the ER alpha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ER alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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