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Flow Cytometry (FC/FACS) (Flow Cytometric analysis of Jurkat cells using EPN2 antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Mouse EPN2 Monoclonal Antibody | anti-EPN2 antibody

EPN2 antibody

Gene Names
EPN2; EHB21
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
EPN2 antibody was purified by affinity chromatography.
Synonyms
EPN2; Monoclonal Antibody; EPN2 antibody; Monoclonal EPN2; Anti-EPN2; EHB21; KIAA1065; EPN 2; EPN-2; anti-EPN2 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1G3
Purity/Purification
EPN2 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
0.5-1 mg/ml (varies by lot)
Sequence Length
61
Applicable Applications for anti-EPN2 antibody
Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:100
FC: 1:100
IHC: 1:150
WB: 1:500-2000
Biological Significance
This gene encodes a protein which interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. The protein is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery. The protein is thought to be involved in clathrin-mediated endocytosis.
Cross-Reactivity
Human, Monkey
Immunogen
EPN2 antibody was raised in Mouse using full length human recombinant protein of human EPN2 produced in HEK293T cell as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Jurkat cells using EPN2 antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Flow Cytometry (FC/FACS) (Flow Cytometric analysis of Jurkat cells using EPN2 antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant EPN2 protein (Right) or empty vector (Left) detected with EPN2 antibody)

Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant EPN2 protein (Right) or empty vector (Left) detected with EPN2 antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of EPN2 protein in paraffin embedded Human endometrium tissue using EPN2 antibody)

Immunohistochemistry (IHC) (Immunohistochemical analysis of EPN2 protein in paraffin embedded Human endometrium tissue using EPN2 antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant EPN2 protein using EPN2 antibody)

Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant EPN2 protein using EPN2 antibody)
Related Product Information for anti-EPN2 antibody
Mouse monoclonal EPN2 antibody
Product Categories/Family for anti-EPN2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
36,729 Da
NCBI Official Full Name
EPN2 protein
NCBI Official Synonym Full Names
epsin 2
NCBI Official Symbol
EPN2
NCBI Official Synonym Symbols
EHB21
NCBI Protein Information
epsin-2
UniProt Protein Name
Epsin-2
UniProt Gene Name
EPN2
UniProt Synonym Gene Names
KIAA1065
UniProt Entry Name
EPN2_HUMAN

NCBI Description

This gene encodes a protein which interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. The protein is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery. The protein is thought to be involved in clathrin-mediated endocytosis. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

epsin 2: Plays a role in the formation of clathrin-coated invaginations and endocytosis. Belongs to the epsin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle

Molecular Function: lipid binding

Biological Process: Notch signaling pathway; in utero embryonic development; embryonic organ development; endocytosis

Research Articles on EPN2

Similar Products

Product Notes

The EPN2 epn2 (Catalog #AAA832940) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's EPN2 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:100 FC: 1:100 IHC: 1:150 WB: 1:500-2000. Researchers should empirically determine the suitability of the EPN2 epn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EPN2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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