Rabbit anti-Human EphB6  Monoclonal Antibody | anti-EphB6  antibody

Rabbit anti Human EphB6  Monoclonal Antibody

Cat. Num. AAA2543876

• Gene Names: EPHB6; HEP
• Reactivity: Human
• Applications: ELISA
• Synonyms: EphB6 ; Monoclonal Antibody; Rabbit anti Human EphB6 Monoclonal Antibody; anti-EphB6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 729

• Applicable Applications for anti-EphB6 antibody: ELISA (EIA)
• Application Notes: ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human EphB6. The detection limit for Human EphB6 is approximately 0.0049 ng/well.
• Immunogen: Recombinant Human EphB6 protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-EphB6  antibody

Ephrins are divided into the ephrin-A (EFNA) class and the ephrin-B (EFNB) class based on their structures and sequence relationships. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB6 is an unusual Eph receptor, lacking catalytic capacity due to alterations in its kinase domain. Interestingly, increased metastatic activity is associated with reduced EphB6 receptor expression in several tumor types, including breast cancer. This emphasizes the potential of EphB6 to act as a suppressor of cancer aggressiveness. EphB6 suppress cancer invasiveness through c-Cbl-dependent signaling, morphologic changes, and cell attachment and indicate that EphB6 may represent a useful prognostic marker and a promising target for therapeutic approaches. EphB6 can both positively and negatively regulate cell adhesion and migration, and suggest that tyrosine phosphorylation of the receptor by an Src family kinase acts as the molecular switch for the functional transition. In addition, Ephrin-B2 may be a physiological ligand for the EphB6 receptor.

NCBI and Uniprot Product Information

• NCBI GI #: 615276339
• NCBI GeneID: 2051
• NCBI GenBank Nucleotide #: NM_001280795.2
• Molecular Weight: 57,498 Da
• NCBI Official Full Name: ephrin type-B receptor 6 isoform b
• NCBI Official Synonym Full Names: EPH receptor B6
• NCBI Official Symbol: EPHB6
• NCBI Official Synonym Symbols: HEP
• NCBI Protein Information: ephrin type-B receptor 6
• UniProt Protein Name: Ephrin type-B receptor 6
• UniProt Gene Name: EPHB6
• UniProt Entry Name: EPHB6_HUMAN

NCBI Description

This gene encodes a member of a family of transmembrane proteins that function as receptors for ephrin-B family proteins. Unlike other members of this family, the encoded protein does not contain a functional kinase domain. Activity of this protein can influence cell adhesion and migration. Expression of this gene is downregulated during tumor progression, suggesting that the protein may suppress tumor invasion and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

EphB6: Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2. Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.10.1; Eph family; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 7q33-q35

Cellular Component: cytosol; extracellular region; plasma membrane

Molecular Function: receptor activity

Biological Process: ephrin receptor signaling pathway