Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western Blot detection against Immunogen (36.63kD).)

Mouse EPHB6 Monoclonal Antibody | anti-EPHB6 antibody

EPHB6 (Ephrin Type-A Receptor 6, Tyrosine-protein Kinase Receptor EPH-6, HEP) APC

Gene Names
EPHB6; HEP
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
EPHB6; Monoclonal Antibody; EPHB6 (Ephrin Type-A Receptor 6; Tyrosine-protein Kinase Receptor EPH-6; HEP) APC; anti-EPHB6 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
5D8
Specificity
Recognizes human EPHB6. Species Crossreactivity: mouse and rat.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-EPHB6 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IHC-P: 3ug/ml
Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa23-122 from EPHB6 (NP_004436) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
DTTGETSEIGWLTYPPGGWDEVSVLDDQRRLTRTFEACHVAGAPPGTGQDNWLQTHFVERRGAQRAHIRLHFSVRACSSLGVSGGTCRETFTLYYRQAEE
Conjugate
APC
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (36.63kD).)

Western Blot (WB) (Western Blot detection against Immunogen (36.63kD).)

Western Blot (WB)

(EPHB6 monoclonal antibody, Western Blot analysis of EPHB6 expression in PC-12.)

Western Blot (WB) (EPHB6 monoclonal antibody, Western Blot analysis of EPHB6 expression in PC-12.)

Western Blot (WB)

(EPHB6 monoclonal antibody. Western Blot analysis of EPHB6 expression in NIH/3T3.)

Western Blot (WB) (EPHB6 monoclonal antibody. Western Blot analysis of EPHB6 expression in NIH/3T3.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to EPHB6 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3ug/ml].)

Immunohistochemistry (IHC) (Immunoperoxidase of monoclonal antibody to EPHB6 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged EPHB6 is ~0.3ng/ml as a capture antibody.)

Testing Data (Detection limit for recombinant GST tagged EPHB6 is ~0.3ng/ml as a capture antibody.)
Product Categories/Family for anti-EPHB6 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
57,498 Da
NCBI Official Full Name
ephrin type-B receptor 6 isoform a
NCBI Official Synonym Full Names
EPH receptor B6
NCBI Official Symbol
EPHB6
NCBI Official Synonym Symbols
HEP
NCBI Protein Information
ephrin type-B receptor 6; human kinase-defective Eph-family receptor protein; tyrosine-protein kinase-defective receptor EPH-6
UniProt Protein Name
Ephrin type-B receptor 6
Protein Family
UniProt Gene Name
EPHB6
UniProt Entry Name
EPHB6_HUMAN

Uniprot Description

EphB6: Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2. Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, tyrosine (receptor); Kinase, protein; Protein kinase, TK; Membrane protein, integral; EC 2.7.10.1; TK group; Eph family

Chromosomal Location of Human Ortholog: 7q33-q35

Cellular Component: integral to plasma membrane; plasma membrane; extracellular region; cytosol

Molecular Function: ephrin receptor activity; receptor activity; ATP binding

Biological Process: axon guidance; ephrin receptor signaling pathway; protein amino acid phosphorylation

Similar Products

Product Notes

The EPHB6 ephb6 (Catalog #AAA6136452) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EPHB6 (Ephrin Type-A Receptor 6, Tyrosine-protein Kinase Receptor EPH-6, HEP) APC reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EPHB6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: 3ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the EPHB6 ephb6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EPHB6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.