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Western Blot (WB)
(Western Blot analysis of EPAS1 expression in transfected 293T cell line by EPAS1 monoclonal antibody (M08), clone 1G2.Lane 1: EPAS1 transfected lysate (Predicted MW: 96.5 KDa).Lane 2: Non-transfected lysate.)
Mouse EPAS1 Monoclonal Antibody | anti-EPAS1 antibody
EPAS1 (Endothelial PAS Domain Protein 1, ECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73) (FITC)
Gene Names
EPAS1; HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73
Applications
Immunofluorescence, Western Blot
Purity
Purified
Synonyms
EPAS1; Monoclonal Antibody; EPAS1 (Endothelial PAS Domain Protein 1; ECYT4; HIF2A; HLF; MOP2; PASD2; bHLHe73) (FITC); Endothelial PAS Domain Protein 1; bHLHe73; anti-EPAS1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1G2
Specificity
Recognizes EPAS1.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Applicable Applications for anti-EPAS1 antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
EPAS1 (NP_001421.2, 28aa-347aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
SKETEVFYELAHELPLPHSVSSHLDKASIMRLAISFLRTHKLLSSVCSENESEAEADQQMDNLYLKALEGFIAVVTQDGDMIFLSENISKFMGLTQVELTGHSIFDFTHPCDHEEIRENLSLKNGSGFGKKSKDMSTERDFFMRMKCTVTNRGRTVNLKSATWKVLHCTGQVKVYNNCPPHNSLCGYKEPLLSCLIIMCEPIQHPSHMDIPLDSKTFLSRHSMDMKFTYCDDRITELIGYHPEELLGRSAYEFYHALDSENMTKSHQNLCTKGQVVSGQYRMLAKHGGYVWLETQGTVIYNPRNLQPQCIMCVNYVLSEI
Conjugate
FITC
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of EPAS1 expression in transfected 293T cell line by EPAS1 monoclonal antibody (M08), clone 1G2.Lane 1: EPAS1 transfected lysate (Predicted MW: 96.5 KDa).Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of EPAS1 expression in transfected 293T cell line by EPAS1 monoclonal antibody (M08), clone 1G2.Lane 1: EPAS1 transfected lysate (Predicted MW: 96.5 KDa).Lane 2: Non-transfected lysate.)
Testing Data
(Detection limit for recombinant GST tagged EPAS1 is 1 ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged EPAS1 is 1 ng/ml as a capture antibody.)
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to EPAS1 on HeLa cell. [antibody concentration 10 ug/ml])
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to EPAS1 on HeLa cell. [antibody concentration 10 ug/ml])
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to EPAS1 on HeLa cell. [antibody concentration 10 ug/ml])
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to EPAS1 on HeLa cell. [antibody concentration 10 ug/ml])
Related Product Information for anti-EPAS1 antibody
Mouse monoclonal antibody raised against a partial recombinant EPAS1.
Product Categories/Family for anti-EPAS1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
96kDa
NCBI Official Full Name
endothelial PAS domain-containing protein 1
NCBI Official Synonym Full Names
endothelial PAS domain protein 1
NCBI Official Symbol
EPAS1
NCBI Official Synonym Symbols
HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73
NCBI Protein Information
endothelial PAS domain-containing protein 1; EPAS-1; HIF2-alpha; HIF-2-alpha; HIF-1alpha-like factor; HIF-1-alpha-like factor; member of PAS protein 2; PAS domain-containing protein 2; hypoxia-inducible factor 2 alpha; hypoxia-inducible factor 2-alpha; ba
UniProt Protein Name
Endothelial PAS domain-containing protein 1
Protein Family
UniProt Gene Name
EPAS1
UniProt Synonym Gene Names
BHLHE73; HIF2A; MOP2; PASD2; EPAS-1; bHLHe73; HLF; HIF-2-alpha; HIF2-alpha
UniProt Entry Name
EPAS1_HUMAN
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Product Notes
The EPAS1 epas1 (Catalog #AAA6178610) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's EPAS1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the EPAS1 epas1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EPAS1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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