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Western Blot (WB) (Western blot detection of Enolase-2 in SHSY-5Y and U87-MG cell lysates using Enolase-2 mouse mAb (1:500 diluted).Predicted band size:47KDa.Observed band size:47KDa.)

Mouse Enolase-2 Monoclonal Antibody | anti-ENO2 antibody

Anti-Enolase-2 Antibody

Gene Names
ENO2; NSE; HEL-S-279
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Synonyms
Enolase-2; Monoclonal Antibody; Anti-Enolase-2 Antibody; 2 phospho D glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; Eno 2; ENO2; ENOG; ENOG_HUMAN; Enolase 2 (gamma; neuronal); Enolase 2; Enolase 2 gamma neuronal; Enolase2; Epididymis secretory protein Li 279; Gamma enolase; Gamma-enolase; HEL S 279; Neural enolase; Neuron specific enolase; Neuron specific gamma enolase; Neuron-specific enolase; Neurone specific enolase; NSE; anti-ENO2 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
lgG1
Form/Format
Purified mouse monoclonal antibody in PBS (pH 7.4) containing with 0.03% Proclin300.
Sequence Length
434
Applicable Applications for anti-ENO2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500
Cellular Location
Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
Tissue Location
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt

Western Blot (WB)

(Western blot detection of Enolase-2 in SHSY-5Y and U87-MG cell lysates using Enolase-2 mouse mAb (1:500 diluted).Predicted band size:47KDa.Observed band size:47KDa.)

Western Blot (WB) (Western blot detection of Enolase-2 in SHSY-5Y and U87-MG cell lysates using Enolase-2 mouse mAb (1:500 diluted).Predicted band size:47KDa.Observed band size:47KDa.)

Western Blot (WB)

(Western blot detection of Enolase-2 in Rat Brain and Mouse Brain lysates using Enolase-2 mouse mAb (1:500 diluted).Predicted band size:47KDa.Observed band size:47KDa.)

Western Blot (WB) (Western blot detection of Enolase-2 in Rat Brain and Mouse Brain lysates using Enolase-2 mouse mAb (1:500 diluted).Predicted band size:47KDa.Observed band size:47KDa.)

ELISA (EIA)

(Observed Enolase-2 levels in CHO-K1 cell lysates transfected with Enolase-2 at different dilution.)

ELISA (EIA) (Observed Enolase-2 levels in CHO-K1 cell lysates transfected with Enolase-2 at different dilution.)
Related Product Information for anti-ENO2 antibody
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47kDa
NCBI Official Full Name
gamma-enolase
NCBI Official Synonym Full Names
enolase 2
NCBI Official Symbol
ENO2
NCBI Official Synonym Symbols
NSE; HEL-S-279
NCBI Protein Information
gamma-enolase
UniProt Protein Name
Gamma-enolase
Protein Family
UniProt Gene Name
ENO2
UniProt Synonym Gene Names
NSE

NCBI Description

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]

Uniprot Description

ENO2: an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.2.1.11; Lyase

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: cytosol; extracellular space

Molecular Function: phosphopyruvate hydratase activity; protein binding

Biological Process: gluconeogenesis

Research Articles on ENO2

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Product Notes

The ENO2 eno2 (Catalog #AAA9231154) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Enolase-2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Enolase-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500. Researchers should empirically determine the suitability of the ENO2 eno2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Enolase-2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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