Mouse anti-Human Endoglin Monoclonal Antibody | anti-END antibody

Endoglin (CD105, ENG, END) (MaxLight 650)

Cat. Num. AAA6222012

• Gene Names: ENG; END; HHT1; ORW1
• Reactivity: Human
• Applications: Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Endoglin; Monoclonal Antibody; Endoglin (CD105; ENG; END) (MaxLight 650); anti-END antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 4C11
• Specificity: Recognizes human ENG.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-END antibody: FLISA, Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa27-658 from human ENG (AAH14271) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: TVHCDLQPVGPERDEVTYTTSQVSKGCVAQAPNAILEVHVLFLEFPTGPSQLELTLQASKQNGTWPREVLLVLSVNSSVFLHLQALGIPLHLAYNSSLVTFQEPPGVNTTELPSFPKTQILEWAAERGPITSAAELNDPQSILLRLGQAQGSLSFCMLEASQDMGRTLEWRPRTPALVRGCHLEGVAGHKEAHILRVLPGHSAGPRTVTVKVELSCAPGDLDAVLILQGPPYVSWLIDANHNMQIWTTGEYSFKIFPEKNIRGFKLPDTPQGLLGEARMLNASIVASFVELPLASIVSLHASSCGGRLQTSPAPIQTTPPKDTCSPELLMSLIQTKCADDAMTLVLKKELVAHLKCTITGLTFWDPSCEAEDRGDKFVLRSAYSSCGMQVSASMISNEAVVNILSSSSPQRKKVHCLNMDSLSFQLGLYLSPHFLQASNTIEPGQQSFVQVRVSPSVSEFLLQLDSCHLDLGPEGGTVELIQGRAAKGNCVSLLSPSPEGDPRFSFLLHFYTVPIPKTGTLSCTVALRPKTGSQDQEVHRTVFMRLNIISPDLSGCTSKGLVLPAVLGITFGAFLIGALLTAALWYIYSHTRSPSKREPVVAVAAPASSESSSTNHSIGSTQSTPCSTSSMA
• Conjugate: MaxLight650
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (95.26kD).)

Western Blot (WB)

(Western Blot analysis of ENG expression in transfected 293T cell line by 126323. Lane 1: ENG transfected lysate (70.6kD). Lane 2: Non-transfected lysate.)

Immunoprecipitation (IP)

(Immunoprecipitation of ENG transfected lysate using 126323 and Protein A Magnetic Bead and immunoblotted with ENG rabbit polyclonal antibody.)

Testing Data

(Detection limit for recombinant GST tagged ENG is ~0.3ng/ml as a capture antibody.)

Related Product Information for anti-END antibody

Endoglin (CD105) is a homodimeric integral membrane glycoprotein comprised of a 95kD subunit. Endoglin is a component of the TGF-B receptor system that functions as an ancillary receptor influencing binding of the Transforming Growth Factor beta (TGF-B) family of ligands to signaling receptors. Endoglin binds to TGF-B1 and TGF-B3 with high affinity, but does not bind TGF-B2. Endoglin is expressed by vascular endothelial cells and syncytiotrophoblasts of placenta and is weakly expressed by stromalfibroblasts. Endoglin is also expressed by U937 cells and by activated macrophages. Endoglin expression is up-regulated on activated endothelium in tissues undergoing angiogenesis, such as in tumors, or in cases of wound healing or dermal inflammation.

Product Categories/Family for anti-END antibody

Antibodies; Endonucleases

NCBI and Uniprot Product Information

• NCBI GI #: 33871100
• NCBI GeneID: 2022
• Molecular Weight: 67,542 Da
• NCBI Official Full Name: Homo sapiens endoglin, mRNA
• NCBI Official Synonym Full Names: endoglin
• NCBI Official Symbol: ENG
• NCBI Official Synonym Symbols: END; HHT1; ORW1
• NCBI Protein Information: endoglin
• Protein Family: Endoglin

NCBI Description

This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]