Mouse anti-Human EGF Receptor Monoclonal Antibody | anti-EGFR antibody

EGF Receptor Antibody

Cat. Num. AAA9215560

• Gene Names: EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunoprecipitation, Immunohistochemistry
• Synonyms: EGF Receptor; Monoclonal Antibody; EGF Receptor Antibody; Purified Mouse Monoclonal Antibody (Mab); Avian erythroblastic leukemia viral (v erb b) oncogene homolog; Cell growth inhibiting protein 40; Cell proliferation inducing protein 61; EGF R; EGFR; EGFR_HUMAN; Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog); Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian); Epidermal growth factor receptor; erbb 1; Erbb; Erbb1; ERBB1; Errp; HER1; mENA; Oncogene ERBB; PIG61; Proto-oncogene c-ErbB-1; Receptor tyrosine protein kinase ErbB 1; Receptor tyrosine-protein kinase ErbB-1; Urogastrone; wa2; Wa5; anti-EGFR antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Form/Format: Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Sequence Length: 1210

• Applicable Applications for anti-EGFR antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: WB ~~ 1:1000; ICC ~~ 1:200; IP ~~ 1:500; IHC ~~ 1:100
• Antigen Source: Human
• Antigen Type: Recombinant Protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot detection of EGFR in HCC827,A549 and MDA-MB-468 cell lysates using EGFR mouse mAb(dilution 1:1000).Predicted band size:134 Kda.Observed band size:175KDa.)

Immunocytochemistry (ICC)

(Immunocytochemistry staining of HeLa cells using EGFR mouse mAb (dilution 1:200).)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of Hela cell lysates using EGFR mouse mAb.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Lung carcinoma using EGF Receptor (3F12) mouse mAb (1/800 dilution) at the Roche Benchmark XT system.)

Related Product Information for anti-EGFR antibody

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.

Product Categories/Family for anti-EGFR antibody

Cancer; Signal Transduction

References

Ullrich A.,et al.Nature 309:418-425(1984). Ilekis J.V.,et al.Mol. Reprod. Dev. 41:149-156(1995). Reiter J.L.,et al.Nucleic Acids Res. 24:4050-4056(1996). Ilekis J.V.,et al.Gynecol. Oncol. 65:36-41(1997). Reiter J.L.,et al.Genomics 71:1-20(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 29725609
• NCBI GeneID: 1956
• NCBI Accession #: NP_005219.2
• NCBI GenBank Nucleotide #: NM_005228.3
• UniProt Accession #: P00533; O00688; O00732; P06268; Q14225; Q68GS5; Q92795; Q9BZS2; Q9GZX1; Q9H2C9; Q9H3C9; Q9UMD7
• Molecular Weight: 134277
• NCBI Official Full Name: epidermal growth factor receptor isoform a
• NCBI Official Synonym Full Names: epidermal growth factor receptor
• NCBI Official Symbol: EGFR
• NCBI Official Synonym Symbols: ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
• NCBI Protein Information: epidermal growth factor receptor
• UniProt Protein Name: Epidermal growth factor receptor
• Protein Family: Epidermal growth factor receptor
• UniProt Gene Name: EGFR
• UniProt Synonym Gene Names: ERBB; ERBB1; HER1
• UniProt Entry Name: EGFR_HUMAN

NCBI Description

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

EGFR: a receptor tyrosine kinase. This is a receptor for epidermal growth factor (EGF) and related growth factors including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30, and vaccinia virus growth factor. EGFR is involved in the control of cell growth and differentiation. It is a single-pass transmembrane tyrosine kinase. Ligand binding to this receptor results in receptor dimerization, autophosphorylation (in trans), activation of various downstream signaling molecules and lysosomal degradation. It can be phosphorylated and activated by Src. Activated EGFR binds the SH2 domain of phospholipase C-gamma (PLC-gamma), activating PLC-gamma-mediated downstream signaling. Phosphorylated EGFR binds Cbl, leading to its ubiquitination and degradation. Grb2 and SHC bind to phospho-EGFR and are involved in the activation of MAP kinase signaling pathways. Phosphorylation on Ser and Thr residues is thought to represent a mechanism for attenuation of EGFR kinase activity. EGFR is overexpressed in breast, head and neck cancers, correlating with poor survival. Activating somatic mutations are seen in lung cancer, corresponding to the minority of patients with strong responses to the EGFR inhibitor Iressa (gefitinib). Mutations and amplifications are also seen in glioblastoma, and upregulation is seen in colon cancer and neoplasms. In xenografts, inhibitors synergize with cytotoxic drugs in the inhibition of many tumor types. Inhibitors include: Iressa/ZD1839, Erbitux, Tarceva, and lapatinib. Four alternatively spliced isoforms have been described.

Protein type: Protein kinase, TK; Membrane protein, integral; Protein kinase, tyrosine (receptor); Tumor suppressor; EC 2.7.10.1; Kinase, protein; TK group; EGFR family

Chromosomal Location of Human Ortholog: 7p12

Cellular Component: extracellular space; endoplasmic reticulum membrane; nuclear membrane; cell surface; focal adhesion; basolateral plasma membrane; integral to membrane; lipid raft; Golgi membrane; membrane; perinuclear region of cytoplasm; early endosome membrane; cytoplasm; plasma membrane; AP-2 adaptor complex; endosome membrane; nucleus; endosome; receptor complex

Molecular Function: identical protein binding; epidermal growth factor receptor activity; nitric-oxide synthase regulator activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; protein phosphatase binding; actin filament binding; protein binding; enzyme binding; transmembrane receptor activity; MAP kinase kinase kinase activity; protein heterodimerization activity; protein-tyrosine kinase activity; ubiquitin protein ligase binding; double-stranded DNA binding; chromatin binding; ATP binding

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; axon guidance; embryonic placenta development; positive regulation of nitric oxide biosynthetic process; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; signal transduction; protein insertion into membrane; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; positive regulation of MAP kinase activity; positive regulation of fibroblast proliferation; cell-cell adhesion; cell surface receptor linked signal transduction; learning and/or memory; hair follicle development; small GTPase mediated signal transduction; positive regulation of cell proliferation; salivary gland morphogenesis; response to stress; regulation of peptidyl-tyrosine phosphorylation; epidermal growth factor receptor signaling pathway; ossification; positive regulation of DNA repair; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; MAPKKK cascade; digestive tract morphogenesis; cell proliferation; positive regulation of protein kinase B signaling cascade; cerebral cortex cell migration; phospholipase C activation; Ras protein signal transduction; insulin receptor signaling pathway; calcium-dependent phospholipase A2 activation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; regulation of nitric-oxide synthase activity; vascular endothelial growth factor receptor signaling pathway; negative regulation of protein catabolic process; positive regulation of DNA replication; positive regulation of epithelial cell proliferation; positive regulation of phosphorylation; negative regulation of apoptosis; positive regulation of cell migration

Disease: Lung Cancer