Mouse anti-Human Ectonucleotide Pyrophosphatase, Phosphodiesterase 7 Monoclonal Antibody | anti-ENPP7 antibody
Ectonucleotide Pyrophosphatase, Phosphodiesterase 7 (ENPP-7, Gm254, Alk-SMase, Enpp7)
Gene Names
ENPP7; NPP-7; E-NPP 7; ALK-SMase
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Ectonucleotide Pyrophosphatase; Phosphodiesterase 7; Monoclonal Antibody; Phosphodiesterase 7 (ENPP-7; Gm254; Alk-SMase; Enpp7); Anti -Ectonucleotide Pyrophosphatase; anti-ENPP7 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
11D63
Specificity
Recognizes human ENPP-7.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS.
Applicable Applications for anti-ENPP7 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA, Western Blot.
Dilution: Direct ELISA: 0.5-1ug/ml
Western Blot: 2ug/ml
Dilution: Direct ELISA: 0.5-1ug/ml
Western Blot: 2ug/ml
Immunogen
Recombinant corresponding to human ENPP-7 expressed in NSO cells (Q6UWV6).
Hybridoma
Myeloma cells with B cells from Balb/c mice.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-ENPP7 antibody
SENPP-7 (ectonucleotide pyrophosphatase/phosphodiesterase 7), also known as alkaline sphingomyelinase (Alk-SMase)is a 60kD GPI-linked membrane glycoprotein expressed in the intestines and in human bile. It shares 30%-36% homology with the NPP family, but is unrelated to neutral or acid SMase. ENPP-7 hydrolyzes dietary sphingomyelin to form ceramide and phosphorylcholine, and may also hydrolyze and inactivate platelet-activating factor (PAF). It is downregulated in some human colorectal carcinomas. The 416aa mature human ENPP-7 shares 80% and 82% aa identity with mouse and rat ENPP-7, respectively.
Product Categories/Family for anti-ENPP7 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,494 Da
NCBI Official Full Name
ectonucleotide pyrophosphatase/phosphodiesterase family member 7
NCBI Official Synonym Full Names
ectonucleotide pyrophosphatase/phosphodiesterase 7
NCBI Official Symbol
ENPP7
NCBI Official Synonym Symbols
NPP-7; E-NPP 7; ALK-SMase
NCBI Protein Information
ectonucleotide pyrophosphatase/phosphodiesterase family member 7; alkaline sphingomyelinase; intestinal alkaline sphingomyelinase; alkaline sphingomyelin phosphodiesterase
UniProt Protein Name
Ectonucleotide pyrophosphatase/phosphodiesterase family member 7
Protein Family
UniProt Gene Name
ENPP7
UniProt Synonym Gene Names
E-NPP 7; NPP-7; Alk-SMase
UniProt Entry Name
ENPP7_HUMAN
Uniprot Description
Function: Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity.
Catalytic activity: Sphingomyelin + H2O = N-acylsphingosine + phosphocholine. Ref.1
Enzyme regulation: Inhibited in a dose dependent manner by ATP, imidazole, orthovanadate and zinc ion. Not inhibited by ADP, AMP and EDTA.
Subcellular location: Membrane; Single-pass type I membrane protein
Potential. Note: Localized at the surface of the microvillar membrane in small intestine enterocytes, and in endosome-like structures situated beneath the microvillar membrane, and in Golgi complex. Ref.1 Ref.6
Tissue specificity: Detected in the colon (at protein level). Expressed in the duodenum, jejunum and liver and at low levels in the ileum. Expression was very low in the esophagus, stomach and colon. Ref.1 Ref.6
Post-translational modification: N-glycosylated; required for activity and transport to the plasma membrane. Ref.8
Miscellaneous: Decreased levels of alkaline sphingomyelin phosphodiesterase may be associated with colon cancer.
Sequence similarities: Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
Research Articles on ENPP7
Similar Products
Product Notes
The ENPP7 enpp7 (Catalog #AAA646912) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Ectonucleotide Pyrophosphatase, Phosphodiesterase 7 (ENPP-7, Gm254, Alk-SMase, Enpp7) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ectonucleotide Pyrophosphatase, Phosphodiesterase 7 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA, Western Blot. Dilution: Direct ELISA: 0.5-1ug/ml Western Blot: 2ug/ml. Researchers should empirically determine the suitability of the ENPP7 enpp7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ectonucleotide Pyrophosphatase, Phosphodiesterase 7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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