Western Blot (WB)
(Western blot analysis of whole cell lysates probed with EBAG9 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP with 10 second exposure. Arrow points to EBAG9 (molecular weight 30 kDa))
Mouse anti-Human, Mouse EBAG9 Monoclonal Antibody | anti-EBAG9 antibody
EBAG9 Antibody with Control Lysate
Gene Names
EBAG9; EB9; PDAF
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
EBAG9; Monoclonal Antibody; EBAG9 Antibody with Control Lysate; Mouse anti EBAG9; EBAG9 Antibody; anti-EBAG9 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
CPTC14
Purity/Purification
Purified by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Liquid in phosphate buffered saline with 0.09% sodium azide (NaN3).
Sequence Length
213
Applicable Applications for anti-EBAG9 antibody
Western Blot (WB)
Application Notes
WB: 1:1000
Western Blotting: Detects a band of approximately 30 kDa in K562 cell lysates.
Western Blotting: Detects a band of approximately 30 kDa in K562 cell lysates.
Immunogen
Recombinant protein of human EBAG9
Lysate Composition
400ug K562 lysate lyophilized in RIPA buffer
Lysate Reconstitution
If using DDT reconstitute the lyophilized lysate with 190mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 10mul 2M DTT.
If using BME reconstitute the lyophilized lysate with 180mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 20u
If using BME reconstitute the lyophilized lysate with 180mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 20u
Preparation and Storage
Antibody: Store undiluted at -20 degree C, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20 degree C.
After reconstitution aliquot and store at -20 degree C for up to 3 months or at -80 degree C for longer term storage. Shelf Life: As supplied, 12 months from date of despatch.
Lysate: Store lyophilized lysate at -20 degree C.
After reconstitution aliquot and store at -20 degree C for up to 3 months or at -80 degree C for longer term storage. Shelf Life: As supplied, 12 months from date of despatch.
Western Blot (WB)
(Western blot analysis of whole cell lysates probed with EBAG9 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP with 10 second exposure. Arrow points to EBAG9 (molecular weight 30 kDa))
Western Blot (WB)
(Western blot analysis of whole cell lysates probed with EBAG9 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP with 10 second exposure. Arrow points to EBAG9 (molecular weight 30 kDa))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
30 kDa
NCBI Official Full Name
Receptor-binding cancer antigen expressed on SiSo cells
NCBI Official Synonym Full Names
estrogen receptor binding site associated, antigen, 9
NCBI Official Symbol
EBAG9
NCBI Official Synonym Symbols
EB9; PDAF
NCBI Protein Information
receptor-binding cancer antigen expressed on SiSo cells
UniProt Protein Name
Receptor-binding cancer antigen expressed on SiSo cells
Protein Family
UniProt Gene Name
EBAG9
UniProt Synonym Gene Names
RCAS1
NCBI Description
This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor, which binds to the estrogen-responsive element found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Alternate splicing results in multiple transcript variants. A pseudogene of this gene has been defined on chromosome 10. [provided by RefSeq, Jul 2013]
Uniprot Description
May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.
Research Articles on EBAG9
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Product Notes
The EBAG9 ebag9 (Catalog #AAA225526) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EBAG9 Antibody with Control Lysate reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EBAG9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000 Western Blotting: Detects a band of approximately 30 kDa in K562 cell lysates. Researchers should empirically determine the suitability of the EBAG9 ebag9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EBAG9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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