Flow Cytometry (FC/FACS)
(Flow Cytometric Analysis of PFA-fixed HeLa cells. E4F1 Mouse Monoclonal Antibody (PCRP-E4F1-2D1) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red). )
Mouse anti-Human E4F1 Monoclonal Antibody | anti-E4F1 antibody
E4F1 Mouse Monoclonal Antibody [Clone PCRP-E4F1-2D1]
Gene Names
E4F1; E4F
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence
Synonyms
E4F1; Monoclonal Antibody; E4F1 Mouse Monoclonal Antibody [Clone PCRP-E4F1-2D1]; E4F transcription factor 1; isoform CRA_b; P120E4F transcription factor; p50E4F; Putative E3 ubiquitin-protein ligase E4F1; RING type E3 ubiquitin transferase E4F1; Transcription factor E4F1; anti-E4F1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse/IgG1
Clone Number
PCRP-E4F1-2D1
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
Purified Ab with BSA and Azide at 200ug/ml
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
Applicable Applications for anti-E4F1 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
FC/FACS: 1-2ug/million cells
IF: 1-2ug/ml
IF: 1-2ug/ml
Immunogen
Recombinant full-length human E4F1 protein
Cellular Localization
Nucleus
Positive Control
HeLa, Jurkat or MCF7 cells.
Hu-chromosome Location
16p13.3
Unigene
513268
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Flow Cytometry (FC/FACS)
(Flow Cytometric Analysis of PFA-fixed HeLa cells. E4F1 Mouse Monoclonal Antibody (PCRP-E4F1-2D1) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red). )
Flow Cytometry (FC/FACS)
(Flow Cytometric Analysis of PFA-fixed HeLa cells. E4F1 Mouse Monoclonal Antibody (PCRP-E4F1-2D1) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red). )
Related Product Information for anti-E4F1 antibody
E4F1 (E4F transcription factor 1), also known as E4F, is a 784 amino acid protein that localizes to both the nucleus and the cytoplasm and contains 9 C2H2-type zinc fingers. Expressed ubiquitously in adult and fetal tissues, E4F1 exists as a homodimer that binds DNA and is thought to act as a transcriptional repressor and may also play a role in cell survival and growth via cell cycle control. Additionally, E4F1 is thought to function as a ubiquitin ligase that mediates the ubiquitination (and subsequent degradation) of target proteins and may be involved in the p53 tumor suppressor pathway. E4F1, which may be post-translationally phosphorylated or sumoylated, is subject to proteolytic cleavage which results in the creation of a short peptide with specific DNA binding capabilities.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
83,496 Da
NCBI Official Full Name
transcription factor E4F1 isoform 2
NCBI Official Synonym Full Names
E4F transcription factor 1
NCBI Official Symbol
E4F1
NCBI Official Synonym Symbols
E4F
NCBI Protein Information
transcription factor E4F1; p50E4F; p120E4F; putative E3 ubiquitin-protein ligase E4F1
UniProt Protein Name
Transcription factor E4F1
Protein Family
UniProt Gene Name
E4F1
UniProt Synonym Gene Names
E4F
UniProt Entry Name
E4F1_HUMAN
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Product Notes
The E4F1 e4f1 (Catalog #AAA4381838) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The E4F1 Mouse Monoclonal Antibody [Clone PCRP-E4F1-2D1] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's E4F1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF). FC/FACS: 1-2ug/million cells IF: 1-2ug/ml. Researchers should empirically determine the suitability of the E4F1 e4f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "E4F1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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