Mouse anti-Human DPP4 / CD26 Monoclonal Antibody | anti-DPP4 / CD26 antibody

Anti-DPP4 / CD26 Antibody (clone 11D7) IHC-plus

Cat. Num. AAA249457

• Gene Names: DPP4; CD26; ADABP; ADCP2; DPPIV; TP103
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein A/G purified
• Synonyms: DPP4 / CD26; Monoclonal Antibody; Anti-DPP4 / CD26 Antibody (clone 11D7) IHC-plus; Mouse Monoclonal [clone 11D7] (IgG1) to Human DPP4 / CD26; Human DPP4 / CD26; ADCP-2; ADABP; Dipeptidyl peptidase IV; Dipeptidyl-peptidase 4; DPPIV; ADCP2; T-cell activation antigen CD26; CD26 antigen; Dipeptidyl peptidase 4; Dipeptidylpeptidase 4; DPP IV; TP103; anti-DPP4 / CD26 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 11D7
• Specificity: Human DPP4 / CD26
• Purity/Purification: Protein A/G purified
• Form/Format: PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 766

• Applicable Applications for anti-DPP4 / CD26 antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flow Cytometry (1:100); IF (1:100); IHC-P (10 ug/ml); WB (1:500)
• Immunogen: DPP4 / CD26 antibody was raised against full length human recombinant protein of human DPP4 (NP_001926) produced in HEK293T cell.
• Immunogen Description: Full length human recombinant protein of human DPP4 (NP_001926) produced in HEK293T cell.
• Immunogen Type: Recombinant protein
• Target Species: Human
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-DPP4 / CD26 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Anti-DPP4 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DPP4.)

Western Blot (WB)

(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPP4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPP4.)

Flow Cytometry (FC/FACS)

(Flow cytometry of HeLa cells, using anti-DPP4 antibody, (Red) compared to a nonspecific negative control antibody (Blue).)

Related Product Information for anti-DPP4 / CD26 antibody

DPP4 Antibody, ADCP-2 Antibody, ADABP Antibody, Dipeptidyl peptidase IV Antibody, Dipeptidyl-peptidase 4 Antibody, DPPIV Antibody, ADCP2 Antibody, T-cell activation antigen CD26 Antibody, CD26 Antibody, CD26 antigen Antibody, Dipeptidyl peptidase 4 Antibody, Dipeptidylpeptidase 4 Antibody, DPP IV Antibody, TP103 Antibody Description: Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion.

NCBI and Uniprot Product Information

• NCBI GI #: 18765694
• NCBI GeneID: 1803
• NCBI Accession #: NP_001926.2
• NCBI GenBank Nucleotide #: NM_001935.3
• UniProt Accession #: P27487; Q53TN1
• Molecular Weight: 88,279 Da
• NCBI Official Full Name: dipeptidyl peptidase 4
• NCBI Official Synonym Full Names: dipeptidyl peptidase 4
• NCBI Official Symbol: DPP4
• NCBI Official Synonym Symbols: CD26; ADABP; ADCP2; DPPIV; TP103
• NCBI Protein Information: dipeptidyl peptidase 4
• UniProt Protein Name: Dipeptidyl peptidase 4
• UniProt Gene Name: DPP4
• UniProt Synonym Gene Names: ADCP2; CD26; ADCP-2; DPP IV
• UniProt Entry Name: DPP4_HUMAN

NCBI Description

The protein encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. [provided by RefSeq, Jul 2008]

Uniprot Description

DPP4: Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF- kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. Belongs to the peptidase S9B family. DPPIV subfamily.

Protein type: Cell adhesion; Protease; Cell surface; Membrane protein, integral; EC 3.4.14.5

Chromosomal Location of Human Ortholog: 2q24.3

Cellular Component: apical plasma membrane; cell surface; endocytic vesicle; focal adhesion; integral to membrane; intercellular canaliculus; lamellipodium; lipid raft; lysosomal membrane; membrane; plasma membrane

Molecular Function: dipeptidyl-peptidase activity; identical protein binding; protease binding; protein binding; protein homodimerization activity; receptor binding; serine-type endopeptidase activity; serine-type peptidase activity; viral receptor activity

Biological Process: behavioral fear response; endothelial cell migration; entry of virus into host cell; positive regulation of cell proliferation; proteolysis; regulation of cell-cell adhesion mediated by integrin; response to hypoxia; T cell activation; T cell costimulation