Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DNMT1 Monoclonal Antibody, Clone 11H8 . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-DNMT1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Nucleus. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) DNMT1. (D) Composite.)
Mouse anti-Human, Mouse DNMT1 Monoclonal Antibody | anti-DNMT1 antibody
Mouse Anti-Human DNMT1 Monoclonal IgG1
Immunohistochemistry: 1-2 ug/mL
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DNMT1 Monoclonal Antibody, Clone 11H8 . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-DNMT1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Nucleus. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) DNMT1. (D) Composite.)
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DNMT1 Monoclonal Antibody, Clone 11H8 . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-DNMT1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Nucleus. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) DNMT1. (D) Composite.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
Uniprot Description
DNMT1: an ubiquitous DNA methyltransferase that methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development, and may play an active role in DNA damage repair. Mediates transcriptional repression by direct binding to HDAC2. Its abundance is reduced to non detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle. Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Three isoforms of the human protein produced by alternative splicing have been described.
Protein type: Methyltransferase; Cell development/differentiation; Amino Acid Metabolism - cysteine and methionine; EC 2.1.1.37; Methyltransferase, DNA; Transcription regulation
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: cell soma; centric heterochromatin; cytoplasm; nucleoplasm; nucleus; protein complex; replication fork
Molecular Function: chromatin binding; DNA (cytosine-5-)-methyltransferase activity; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; DNA binding; DNA-methyltransferase activity; double-stranded DNA binding; estrogen receptor binding; histone deacetylase binding; methyl-CpG binding; protein binding; protein domain specific binding; RNA binding; transcription factor binding; unmethylated CpG binding; zinc ion binding
Biological Process: aging; brain development; chromatin modification; cytosine methylation within a CG sequence; DNA methylation; DNA methylation on cytosine; gene expression; gene silencing; maintenance of DNA methylation; negative regulation of gene expression, epigenetic; negative regulation of histone H3-K9 methylation; negative regulation of transcription from RNA polymerase II promoter; neuron differentiation; positive regulation of histone H3-K4 methylation; regulation of cell proliferation; regulation of gene expression, epigenetic; response to activity; response to caffeine; response to estradiol stimulus; response to ethanol; response to heat; response to ionizing radiation; response to lipopolysaccharide; response to testosterone stimulus; response to toxin; response to vitamin A; S-adenosylhomocysteine metabolic process; S-adenosylmethioninamine metabolic process; S-adenosylmethionine metabolic process; transcription, DNA-dependent
Disease: Cerebellar Ataxia, Deafness, And Narcolepsy, Autosomal Dominant; Neuropathy, Hereditary Sensory, Type Ie
Research Articles on DNMT1
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Product Notes
The DNMT1 dnmt1 (Catalog #AAA807263) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human DNMT1 Monoclonal IgG1 reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DNMT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: 2-4 ug/mL Immunohistochemistry: 1-2 ug/mL. Researchers should empirically determine the suitability of the DNMT1 dnmt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNMT1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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