Western Blot (WB)
(Western blot analysis of whole cell lysates probed with DNA PKcs antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemicDoc„¢ MP with 41 second exposure. Arrow points to DNA PKcs (molecular weight >350 kDa))
Mouse anti-Human DNA PKcs Monoclonal Antibody | anti-PRKDC antibody
Mouse anti DNA PKcs
Western Blotting: Detects a band of approximately >350 kDa in MOLT-4 cell lysates.
Western Blot (WB)
(Western blot analysis of whole cell lysates probed with DNA PKcs antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemicDoc„¢ MP with 41 second exposure. Arrow points to DNA PKcs (molecular weight >350 kDa))
Western Blot (WB)
(Western blot analysis of whole cell lysates probed with DNA PKcs antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemicDoc„¢ MP with 41 second exposure. Arrow points to DNA PKcs (molecular weight >350 kDa))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010]
Uniprot Description
Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect mechanism. Interacts with CRY1 and CRY2; negatively regulates CRY1 phosphorylation. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.
Research Articles on PRKDC
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Product Notes
The PRKDC prkdc (Catalog #AAA225490) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti DNA PKcs reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNA PKcs can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000 Western Blotting: Detects a band of approximately >350 kDa in MOLT-4 cell lysates. Researchers should empirically determine the suitability of the PRKDC prkdc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA PKcs, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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