Hamster DL1 Monoclonal Antibody | anti-DL1 antibody

DL1 antibody (PE)

Cat. Num. AAA833622

• Gene Names: DLL1; DL1; Delta; DELTA1
• Applications: Flow Cytometry, Functional Assay, Immunocytochemistry
• Synonyms: DL1; Monoclonal Antibody; DL1 antibody (PE); Monoclonal DL1; Anti-DL1; DL-1; DL 1; anti-DL1 antibody

• Host: Hamster
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: HMD1-5
• Form/Format: Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
• Concentration: 200 ug/ml (varies by lot)
• Sequence Length: 723

• Applicable Applications for anti-DL1 antibody: Flow Cytometry (FC/FACS), Immunocytochemistry (ICC)
• Application Notes: FC: <= 0.5 ug/test; ; A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Biological Significance: The HMD1-5 monoclonal antibody reacts with mouse Delta-like 1, one of five type I transmembrane proteins that serves as a Notch receptor ligand. Upon binding the Notch receptor, Delta-like 1 undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases an intracellular fragment whose biological function remains controversial. Delta-like 1 is expressed by thymic stromal cells, as well as on macrophages, dendritic cells, and stromal cells in the spleen. This protein is also expressed in nonhematopoietic tissues such as the lung and brain. Delta-like 1 has been linked to B cell development and differentiation, especially marginal zone and plasma cells, regulation of splenic dendritic cells, and leukemogenesis.
• Tag/Conjugate: PE
• Preparation and Storage: Store at 2-8 degree C. Do not freeze. Protect from light.

Flow Cytometry (FC/FACS)

(Staining of CD8+CD11c+ C57BL/6 splenocytes with 0.125 ug of Armenian Hamster IgG Isotype Control (PE) (blue histogram) or 0.125 ug of DLL1 antibody (PE) (purple histogram). Total viable cells were used for analysis.)

Related Product Information for anti-DL1 antibody

Armenian Hamster monoclonal Dll1 antibody (PE)

Product Categories/Family for anti-DL1 antibody

Immunology; Conjugated Monoclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 110735443
• NCBI GeneID: 28514
• NCBI Accession #: NP_005609.3
• NCBI GenBank Nucleotide #: NM_005618.3
• Molecular Weight: 27,355 Da
• NCBI Official Full Name: delta-like protein 1
• NCBI Official Synonym Full Names: delta-like 1 (Drosophila)
• NCBI Official Symbol: DLL1
• NCBI Official Synonym Symbols: DL1; Delta; DELTA1
• NCBI Protein Information: delta-like protein 1
• UniProt Protein Name: Delta-like protein 1
• UniProt Gene Name: DLL1
• UniProt Synonym Gene Names: Delta1; H-Delta-1
• UniProt Entry Name: DLL1_HUMAN

NCBI Description

DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may play a role in cell-to-cell communication. [provided by RefSeq, Jul 2008]

Uniprot Description

DLL1: Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. Interacts with Notch receptors. Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.

Protein type: Cell development/differentiation; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6q27

Cellular Component: integral to plasma membrane; extracellular region; plasma membrane; cytoplasmic vesicle

Molecular Function: protein binding; calcium ion binding; Notch binding

Biological Process: regulation of cell adhesion; Notch signaling pathway; negative regulation of myeloid cell differentiation; negative regulation of interleukin-10 production; Notch receptor processing; cell fate determination; compartment specification; somite specification; cell-cell signaling; negative regulation of auditory receptor cell differentiation; hemopoiesis; positive regulation of transcription from RNA polymerase II promoter; heart looping; cell differentiation; determination of left/right symmetry; inner ear development; positive regulation of Notch signaling pathway