Mouse DKK1 Monoclonal Antibody | anti-DKK1 antibody

DKK1 (Dickkopf Homolog 1 (Xenopus laevis), DKK-1, SK) (HRP)

Cat. Num. AAA6181484

• Gene Names: DKK1; SK; DKK-1
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified
• Synonyms: DKK1; Monoclonal Antibody; DKK1 (Dickkopf Homolog 1 (Xenopus laevis); DKK-1; SK) (HRP); Dickkopf Homolog 1 (Xenopus laevis); SK; anti-DKK1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 2A5
• Specificity: Recognizes DKK1.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
• Sequence Length: 266

• Applicable Applications for anti-DKK1 antibody: Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: DKK1 (AAH01539.1, 1aa-266aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Immunoperoxidase of monoclonal antibody to DKK1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to DKK1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])

Western Blot (WB)

(DKK1 monoclonal antibody (M11), clone 2A5 Western Blot analysis of DKK1 expression in U-2 OS (Cat # L022V1).)

Immunoprecipitation (IP)

(Immunoprecipitation of DKK1 transfected lysate using anti-DKK1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with DKK1 MaxPab rabbit polyclonal antibody.)

Product Categories/Family for anti-DKK1 antibody

Antibodies; Dickkopf Proteins

References

1. Genetic grouping of medulloblastomas by representative markers in pathologic diagnosis. Min HS, Lee JY, Kim SK, Park SHTransl Oncol. 2013 Jun 1;6(3):265-72. Print 2013 Jun. 2.RNA interference?mediated targeting of DKK1 gene expression in Ishikawa endometrial carcinoma cells causes increased tumor cell invasion and migration.Yi N, Liao QP, Li ZH, Xie BJ, Hu YH, Yi W, Liu M.ONCOLOGY LETTERS 6: 756-762, 2013 3.Molecular subgroups of medulloblastoma: the current consensus. Taylor MD, Northcott PA, Korshunov A, Remke M, Cho YJ, Clifford SC, Eberhart CG, Parsons DW, Rutkowski S, Gajjar A, Ellison DW, Lichter P, Gilbertson RJ, Pomeroy SL, Kool M, Pfister SM.Acta Neuropathol. 2011 Dec 2. 4.Prokineticin 1 induces Dickkopf 1 expression and regulates cell proliferation and decidualization in the human endometrium. Macdonald LJ, Sales KJ, Grant V, Brown P, Jabbour HN, Catalano RD.Mol Hum Reprod. 2011 May 5. 5. Carbonic anhydrase IX (CA9) modulates tumor-associated cell migration and invasion.Shin HJ, Rho SB, Jung DC, Han IO, Oh ES, Kim JY.J Cell Sci. 2011 Apr 1;124(Pt 7):1077-87. Epub 2011 Mar 1. 6.Bone and bone marrow pro-osteoclastogenic cytokines are up-regulated in osteoporosis fragility fractures.D'Amelio P, Roato I, D'Amico L, Veneziano L, Suman E, Sassi F, Bisignano G, Ferracini R, Gargiulo G, Castoldi F, Pescarmona GP, Isaia GC.Osteoporos Int. 2010 Nov 30.

NCBI and Uniprot Product Information

• NCBI GI #: 16306720
• NCBI GeneID: 22943
• NCBI Official Full Name: Dickkopf homolog 1 (Xenopus laevis)
• NCBI Official Synonym Full Names: dickkopf WNT signaling pathway inhibitor 1
• NCBI Official Symbol: DKK1
• NCBI Official Synonym Symbols: SK; DKK-1
• NCBI Protein Information: dickkopf-related protein 1
• Protein Family: Dickkopf-related protein

NCBI Description

This gene encodes a member of the dickkopf family of proteins. Members of this family are secreted proteins characterized by two cysteine-rich domains that mediate protein-protein interactions. The encoded protein binds to the LRP6 co-receptor and inhibits beta-catenin-dependent Wnt signaling. This gene plays a role in embryonic development and may be important in bone formation in adults. Elevated expression of this gene has been observed in numerous human cancers and this protein may promote proliferation, invasion and growth in cancer cell lines. [provided by RefSeq, Sep 2017]