Mouse anti-Human Dihydropyrimidine Dehydrogenase Monoclonal Antibody | anti-DPYD antibody

Dihydropyrimidine Dehydrogenase (DPD, DPYD, DHP, DHPDHase, Dihydrothymine Dehydrogenase, Dihydrouracil Dehydrogenase, MGC70799, MGC132008)

Cat. Num. AAA6006150

• Gene Names: DPYD; DHP; DPD; DHPDHASE
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Dihydropyrimidine Dehydrogenase; Monoclonal Antibody; Dihydropyrimidine Dehydrogenase (DPD; DPYD; DHP; DHPDHase; Dihydrothymine Dehydrogenase; Dihydrouracil Dehydrogenase; MGC70799; MGC132008); Anti -Dihydropyrimidine Dehydrogenase (DPD; anti-DPYD antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 7D4
• Specificity: Recognizes human DPYD.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MAPVLSKDSADIESILALNPRTQTHATLCSTSAKKLDKKHWKRNPDKNCFNCEKLENNFDDIKHTTLGERGALREAMRCLKCADAPCQKSCPTNLDIKSFITSIANKNYY

• Applicable Applications for anti-DPYD antibody: ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, ELISA and Western Blot.; Dilution: Immunofluorescence: 10ug/ml
• Immunogen: Partial recombinant corresponding to aa1-110 from human DPYD (NP_000101) with GST tag. MW of the GST tag alone is 26kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (37.84kD).)

Western Blot (WB)

(DPYD monoclonal antibody, Western Blot analysis of DPYD expression in HeLa.)

Western Blot (WB)

(DPYD monoclonal antibody. Western Blot analysis of DPYD expression in Hela NE.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to DPYD on HeLa cell. [antibody concentration 10ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged DPYD is ~0.1ng/ml as a capture antibody.)

Related Product Information for anti-DPYD antibody

The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-DPYD antibody

Antibodies; Abs to Enzymes; Dehydrogenase

NCBI and Uniprot Product Information

• NCBI GI #: 693912
• NCBI GeneID: 1806
• UniProt Accession #: Q12882; Q16694; Q16761; Q32NB0; Q96HL6; Q96TH1; A2RRQ2; A2RRQ3; A8K5A2; A8MWG9; B1AN21; E9PFN1
• Molecular Weight: 111,401 Da
• NCBI Official Full Name: dihydropyrimidine dehydrogenase
• NCBI Official Synonym Full Names: dihydropyrimidine dehydrogenase
• NCBI Official Symbol: DPYD
• NCBI Official Synonym Symbols: DHP; DPD; DHPDHASE
• NCBI Protein Information: dihydropyrimidine dehydrogenase [NADP(+)]; dihydropyrimidine dehydrogenase [NADP(+)]; dihydrouracil dehydrogenase; dihydrothymine dehydrogenase
• UniProt Protein Name: Dihydropyrimidine dehydrogenase [NADP(+)]
• Protein Family: Dihydropyrimidine dehydrogenase
• UniProt Gene Name: DPYD
• UniProt Synonym Gene Names: DHPDHase; DPD
• UniProt Entry Name: DPYD_HUMAN

NCBI Description

The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

DPYD: Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil. Homodimer. Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells. Belongs to the dihydropyrimidine dehydrogenase family.

Protein type: Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Nucleotide Metabolism - pyrimidine; Other Amino Acids Metabolism - beta-alanine; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - other enzymes; EC 1.3.1.2

Chromosomal Location of Human Ortholog: 1p22

Cellular Component: cytoplasm; cytosol

Molecular Function: protein homodimerization activity; FAD binding; metal ion binding; dihydroorotate dehydrogenase activity; 4 iron, 4 sulfur cluster binding; dihydropyrimidine dehydrogenase (NADP+) activity; NADP binding

Biological Process: pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; uracil catabolic process; UMP biosynthetic process; thymine catabolic process; beta-alanine biosynthetic process; purine base catabolic process; pyrimidine base catabolic process; thymidine catabolic process

Disease: Dihydropyrimidine Dehydrogenase Deficiency