Mouse anti-Human DHX9 Monoclonal Antibody | anti-DHX9 antibody

Mouse monoclonal antibody Anti-Human DHX9

Cat. Num. AAA120267

• Gene Names: DHX9; LKP; RHA; DDX9; NDH2; NDHII
• Reactivity: Human
• Applications: Dot Blot
• Synonyms: DHX9; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human DHX9; Homo sapiens DEAH (Asp-Glu-Ala-His) box polypeptide 9 (DHX9); transcript variant 1; LKP; RHA; DDX9; NDHII; NDH II; anti-DHX9 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 2274D5a
• Concentration: 100 ug/ml (1.0 ml) (varies by lot)
• Sequence Length: 4543

• Applicable Applications for anti-DHX9 antibody: Dot Blot (DB)
• Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Sterility: Filtered through a 0.22 um membrane.

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-DHX9 monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Related Product Information for anti-DHX9 antibody

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein with RNA helicase activity. It may participate in melting of DNA:RNA hybrids, such as those that occur during transcription, and may play a role in X-linked gene expression. It contains 2 copies of a double-stranded RNA-binding domain, a DEXH core domain and an RGG box. The RNA-binding domains and RGG box influence and regulate RNA helicase activity. [NCBI Entrez Gene Summary]

NCBI and Uniprot Product Information

• NCBI GI #: 289063428
• NCBI GeneID: 1660
• NCBI Accession #: NP_001348.2
• NCBI GenBank Nucleotide #: NM_001357
• Molecular Weight: 24,336 Da
• NCBI Official Full Name: Homo sapiens DEAH (Asp-Glu-Ala-His) box helicase 9 (DHX9), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: DEAH (Asp-Glu-Ala-His) box helicase 9
• NCBI Official Symbol: DHX9
• NCBI Official Synonym Symbols: LKP; RHA; DDX9; NDH2; NDHII
• NCBI Protein Information: ATP-dependent RNA helicase A; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9; DEAH (Asp-Glu-Ala-His) box polypeptide 9; DEAH box protein 9; leukophysin; nuclear DNA helicase II
• UniProt Protein Name: ATP-dependent RNA helicase A
• Protein Family: ATP-dependent RNA helicase
• UniProt Gene Name: DHX9
• UniProt Entry Name: DHX9_HUMAN

NCBI Description

This gene encodes a member of the DEAH-containing family of RNA helicases. The encoded protein is an enzyme that catalyzes the ATP-dependent unwinding of double-stranded RNA and DNA-RNA complexes. This protein localizes to both the nucleus and the cytoplasm and functions as a transcriptional regulator. This protein may also be involved in the expression and nuclear export of retroviral RNAs. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 11 and 13.[provided by RefSeq, Feb 2010]

Uniprot Description

DDX9: a helicase that unwinds double-stranded DNA and RNA in a 3' to 5' direction. Interacts with the RNA-induced silencing complex (RISC) in human cells and functions in RISC loading. Generates multiple secondary structures that influence RNA-binding proteins. Has a dsRNA binding motif at the N-terminus, RGG-box at the carboxyl terminus, and a bidirectional nuclear transport domain of 110 amino acids at the carboxyl terminus. May play a role in X-linked gene expression.

Protein type: Nucleolus; RNA processing; RNA splicing; Nuclear receptor co-regulator; EC 3.6.4.13; RNA-binding; Helicase; Spliceosome

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: nucleoplasm; centrosome; membrane; cytoplasm; nucleolus; cytosol; nucleus; ribonucleoprotein complex

Molecular Function: ATP-dependent DNA helicase activity; protein binding; DNA binding; ATP binding; RNA helicase activity; ATP-dependent RNA helicase activity

Biological Process: circadian rhythm; osteoblast differentiation; nuclear mRNA splicing, via spliceosome; RNA processing; RNA splicing; positive regulation of interferon type I production; innate immune response; gene expression; DNA duplex unwinding