Mouse anti-Human Cytokeratin 7/KRT7 Monoclonal Antibody | anti-KRT7 antibody

Anti-Cytokeratin 7/KRT7 Antibody, Mouse Monoclonal

Cat. Num. AAA8100233

• Gene Names: KRT7; K7; CK7; SCL; K2C7
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Protein A
• Synonyms: Cytokeratin 7/KRT7; Monoclonal Antibody; Anti-Cytokeratin 7/KRT7 Antibody; Mouse Monoclonal; KRT7/ Cytokeratin-7 Antibody; Mouse MAb; keratin 7; type II; Anti-CK7 Antibody; Anti-K2C7 Antibody; Anti-K7 Antibody; Anti-SCL Antibody; anti-KRT7 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG3
• Clone Number: 02
• Specificity: Human KRT7/ Cytokeratin-7
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-KRT7 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:50-1:200
• Immunogen: A synthetic peptide corresponding to the center region of the Human KRT7/ Cytokeratin-7
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the center region of the Human KRT7/Cytokeratin-7. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human KRT7 in human breast carcinoma with mouse monoclonal antibody (1:50, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-KRT7 antibody

KRT7 (Keratin 7) is a Protein Coding gene. The protein encoded by this gene is a member of the keratin gene family. One AS, designated Homo sapiens keratin 7 (KRT7-AS), was selected due to its marked upregulation and concordant expression with its cognate sense counterpart, KRT7, in GC tissues and cell lines. KRT7-AS formed an RNA-RNA hybrid with KRT7 and controlled KRT7 expression at both the mRNA and the post-transcriptional levels. Moreover, forced overexpression of the KRT7-overlapping region (OL) of KRT7-AS (but not its non-KRT7-OL portions) increased keratin 7 protein levels in cells. Finally, forced overexpression of full-length KRT7-AS or OL KRT7-AS (but not its non-KRT7-OL regions) promoted GC cell proliferation and migration.

NCBI and Uniprot Product Information

• NCBI GI #: 67782365
• NCBI GeneID: 3855
• NCBI Accession #: NP_005547.3
• NCBI GenBank Nucleotide #: NM_005556.3
• UniProt Accession #: P08729; Q92676; Q9BUD8; Q9Y3R7
• Molecular Weight: 51,386 Da
• NCBI Official Full Name: keratin, type II cytoskeletal 7
• NCBI Official Synonym Full Names: keratin 7
• NCBI Official Symbol: KRT7
• NCBI Official Synonym Symbols: K7; CK7; SCL; K2C7
• NCBI Protein Information: keratin, type II cytoskeletal 7; CK-7; keratin-7; sarcolectin; cytokeratin 7; cytokeratin-7; type-II keratin Kb7; type II mesothelial keratin K7; keratin, 55K type II cytoskeletal; keratin, simple epithelial type I, K7
• UniProt Protein Name: Keratin, type II cytoskeletal 7
• Protein Family: Keratin
• UniProt Gene Name: KRT7
• UniProt Synonym Gene Names: SCL; CK-7; K7
• UniProt Entry Name: K2C7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]

Uniprot Description

K7: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: cytoplasm; keratin filament; intermediate filament; nucleus

Molecular Function: protein binding; structural molecule activity

Biological Process: viral reproduction