Mouse anti-Bovine, Pig CYTOKERATIN 7/17 Monoclonal Antibody | anti-KRT17 antibody

MOUSE ANTI HUMAN CYTOKERATIN 7/17

Cat. Num. AAA214467

• Gene Names: KRT17; PC; K17; PC2; PCHC1
• Reactivity: Bovine, Pig
• Applications: Immunoprecipitation, Immunohistochemistry, Western Blot
• Synonyms: CYTOKERATIN 7/17; Monoclonal Antibody; MOUSE ANTI HUMAN CYTOKERATIN 7/17; anti-KRT17 antibody

• Host: Mouse
• Reactivity: Bovine, Pig
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: C-46
• Specificity: This item specifically recognises a unique epitope shared by the intermediate filament keratins, cytokeratin 7 and cytokeratin 17, a 54kDa type II basic cytokeratin, and 46kDa type I acidic cytokeratin respectively. Cytokeratins are located in the intracytoplasmic cytoskeleton of epithelial tissues and their neoplasms, including the lungs, ovary and breast. Each keratin filament consists of at least one type I and one type II keratin chain, which then assemble to form a cytoskeletal network, extending from the surface of the nucleus to the cell membrane. The expression of a particular type of cytokeratin depends mainly on the type of epithelium and the development stage of the cell, including its corresponding malignant state. Immunohistochemical analysis of cytokeratin profiles is therefore a valuable tool for characterization, and this particular antibody could be useful for the evaluation of some stratified epithelia which express cytokeratin 17, but not cytokeratin 7. This item is reported as suitable for use in immunocytochemistry, see Bartek, J. et al. for details.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 432

• Applicable Applications for anti-KRT17 antibody: Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Western Blot: This item detects bands of approximately 54kDa and 46kDa in HeLa cytoskeleton cell lysates under reducing conditions.; Immunohistology - Paraffin: Minimum Dilution: 20; Maximum Dilution: 30ug/ml; Application Note: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.; Western Blotting: Minimum Dilution: 2; Maximum Dilution: 3ug/ml
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Immunogen: HeLa cell cytoskeleton preparation.; Histology Positive Control Tissue
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-KRT17 antibody

MBS214467 specifically recognises a unique epitope shared by the intermediate filament keratins, cytokeratin 7 and cytokeratin 17, a 54kDa type II basic cytokeratin, and 46kDa type I acidic cytokeratin respectively. Cytokeratins are located in the intracytoplasmic cytoskeleton of epithelial tissues and their neoplasms, including the lungs, ovary and breast. Each keratin filament consists of at least one type I and one type II keratin chain, which then assemble to form a cytoskeletal network, extending from the surface of the nucleus to the cell membrane. The expression of a particular type of cytokeratin depends mainly on the type of epithelium and the development stage of the cell, including its corresponding malignant state. Immunohistochemical analysis of cytokeratin profiles is therefore a valuable tool for identification and characterization, and this particular antibody could be useful for the evaluation of some stratified epithelia which express cytokeratin 17, but not cytokeratin 7. MBS214467 is reported as suitable for use in immunocytochemistry, see Bartek, J. et al. for details.

NCBI and Uniprot Product Information

• NCBI GI #: 4557701
• NCBI GeneID: 3872
• NCBI Accession #: NP_000413.1
• NCBI GenBank Nucleotide #: NM_000422.2
• Molecular Weight: 48,106 Da
• NCBI Official Full Name: keratin, type I cytoskeletal 17
• NCBI Official Synonym Full Names: keratin 17, type I
• NCBI Official Symbol: KRT17
• NCBI Official Synonym Symbols: PC; K17; PC2; PCHC1
• NCBI Protein Information: keratin, type I cytoskeletal 17; 39.1; CK-17; cytokeratin-17; keratin 17 epitope S1; keratin 17 epitope S2; keratin 17 epitope S4
• UniProt Protein Name: Keratin, type I cytoskeletal 17
• Protein Family: Keratin
• UniProt Gene Name: KRT17
• UniProt Synonym Gene Names: CK-17; K17
• UniProt Entry Name: K1C17_HUMAN

NCBI Description

This gene encodes the type I intermediate filament chain keratin 17, expressed in nail bed, hair follicle, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex. [provided by RefSeq, Aug 2008]

Uniprot Description

K17: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K6.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoplasm; intermediate filament

Molecular Function: MHC class II receptor activity; protein binding; structural constituent of cytoskeleton; MHC class II protein binding

Biological Process: positive regulation of hair follicle development; epidermis development; hair follicle morphogenesis; positive regulation of translation; keratinization; intermediate filament organization; signal transduction; positive regulation of cell growth

Disease: Steatocystoma Multiplex; Pachyonychia Congenita 2