Mouse anti-Human Cytokeratin 5 + 14 Monoclonal Antibody
Cytokeratin 5 + 14
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
PurifiedPurified
Synonyms
Cytokeratin 5 + 14; Monoclonal Antibody; Anti -Cytokeratin 5 + 14; anti-Cytokeratin 5 + 14 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1 & IgG3
Clone Number
8J1213
Specificity
This antibody cocktail reacts with cytokeratin 5 intermediate filament protein as well as to 50kD cytokeratin protein designated cytokeratin 14. Basal cells of human epidermis express acidic cytokeratin 14 and basic cytokeratin 5. Cytokeratin 5 is a 58kD protein, which is closely related to cytokeratin 6. Cytokeratin 5 and 6 are expressed in basal cell epithelioma, basal cells of prostate, urothelium, vagina, squamous cell carcinomas of skin, tongue, epiglottis and rectal-anal region. Cytokeratin 5 can be useful in the distinction of mesotheliomas from most adenocarcinomas. Cytokeratin 14 belongs to the type A (acidic) subfamily of low MW cytokeratins and exists in combination with cytokeratin 15. This antibody can be useful to distinguish stratified epithelial cells from simple epithelial cells. Species Crossreactivity: This antibody is reactive against human.
Purity/Purification
Purified
Purified
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Applicable Applications for anti-Cytokeratin 5 + 14 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry (Fr) and Immunocytochemistry.
Immunogen
XXXX: prokaryotic recombinant protein corresponding to 103 amino acid portion of C-terminal region of the human cytokeratin 5 molecule. LLLLL: Synthetic peptide of the extreme c-terminal of human keratin-14.
Cellular Localization
Cytoplasmic
Positive Control
Skin
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Cytokeratin 5 + 14 antibody
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70kD and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis.
Product Categories/Family for anti-Cytokeratin 5 + 14 antibody
Similar Products
Product Notes
The Cytokeratin 5 + 14 (Catalog #AAA603579) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Cytokeratin 5 + 14 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin 5 + 14 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF). Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry (Fr) and Immunocytochemistry. Researchers should empirically determine the suitability of the Cytokeratin 5 + 14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 5 + 14, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
