Immunofluorescence/Immunocytochemistry (IF/ICC)
Mouse Cytokeratin 3 Monoclonal Antibody
Mouse Anti-Cytokeratin 3 [AE5] Antibody
Reactivity
Rabbit, Cow, Human
Applications
Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
Purity
Protein G purified
Synonyms
Cytokeratin 3; Monoclonal Antibody; Mouse Anti-Cytokeratin 3 [AE5] Antibody; CK3 antibody; FLJ95909 antibody; K3 antibody; 65 kDa cytokeratin antibody; CK 3 antibody; Cytokeratin3 antibody; Keratin 3 antibody; Keratin type II cytoskeletal 3 antibody; Keratin3 antibody; KRT 3 antibody; KRT3 antibody;; anti-Cytokeratin 3 antibody
Host
Mouse
Reactivity
Rabbit, Cow, Human
Clonality
Monoclonal
Isotype
Ig
Clone Number
AE5
Purity/Purification
Protein G purified
Form/Format
Purified antibody containing PBS 0.1% sodium azide;
Concentration
0.7mg/ml (varies by lot)
Applicable Applications for anti-Cytokeratin 3 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Optimal antibody dilution should be determined by titration, however as guideline try at WB: 1/1000 - 1/3000. Predicted molecular weight: 65 kDa. Flow Cyt: Use 2ug for 106 cells.
Target Antigen
Cytokeratin 3
Immunogen
Rabbit corneal epithelial keratins
Myeloma/fusion Partners
P3 x 63 Ag8 myeloma cells
Research Areas
Cell Biology
Immunofluorescence/Immunocytochemistry (IF/ICC)
Immunofluorescence/Immunocytochemistry (IF/ICC)
References
Shahdadfar A et al. Ex vivo expanded autologous limbal epithelial cells on amniotic membrane using a culture medium with human serum as single supplement.Exp Eye Res. 2012 Apr;97(1):1-9. doi: 10.1016/j.exer.2012.01.013. Epub 2012 Feb 7. IHC ; Human PMID: 22342952 Hanson C et al. Transplantation of human embryonic stem cells onto a partially wounded human cornea in vitro. Acta Ophthalmol : (2012) PMID: 22280565 Priya CG et al. Adult human buccal epithelial stem cells: identification, ex-vivo expansion, and transplantation for corneal surface reconstruction.Eye (Lond). 2011 Dec;25(12):1641-9. doi: 10.1038/eye.2011.230. Epub 2011 Sep 23.IHC ; Human PMID: 21941360 Liao H et al. Development of Allele-Specific Therapeutic siRNA in Meesmann Epithelial Corneal Dystrophy. PLoS One 6:e28582 (2011). ICC/IF ; Human PMID: 22174841 Utheim TP et al. Comparison of the histology, gene expression profile, and phenotype of cultured human limbal epithelial cells from different limbal regions.Invest Ophthalmol Vis Sci. 2009 Nov;50(11):5165-72. doi: 10.1167/iovs.08-2884. Epub 2009 Jul 2.IHC ; Human PMID: 19578011 Raeder S et al. Effects of organ culture and Optisol-GS storage on structural integrity, phenotypes, and apoptosis in cultured corneal epithelium.Invest Ophthalmol Vis Sci. 2007 Dec;48(12):5484-93.IHC ; Human PMID: 18055796 Raeder S et al. Effect of limbal explant orientation on the histology, phenotype, ultrastructure and barrier function of cultured limbal epithelial cells.Acta Ophthalmol Scand. 2007 Jun;85(4):377-86. Epub 2007 Jan 31. IHC ; Human PMID: 17263847 Rodrigues M et al. Suprabasal expression of a 64-kilodalton keratin (no. 3) in developing human corneal epithelium. Differentiation 34:60-7 (1987) PMID: 2440750 Cooper D & Sun TT Monoclonal antibody analysis of bovine epithelial keratins. Specific pairs as defined by coexpression. J Biol Chem 261:4646-54PMID:2420789 Schermer A et al. Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells. J Cell Biol 103:49-62 (1986) PMID: 2424919
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Product Notes
The Cytokeratin 3 (Catalog #AAA375045) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Cytokeratin 3 [AE5] Antibody reacts with Rabbit, Cow, Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin 3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS). Optimal antibody dilution should be determined by titration, however as guideline try at WB: 1/1000 - 1/3000. Predicted molecular weight: 65 kDa. Flow Cyt: Use 2ug for 106 cells. Researchers should empirically determine the suitability of the Cytokeratin 3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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