Mouse anti-Human, Cat Cytokeratin 10/13 Monoclonal Antibody | anti-KRT10 antibody

Cytokeratin 10/13 Mouse Monoclonal Antibody

Cat. Num. AAA438984

• Gene Names: KRT10; BIE; EHK; K10; KPP; BCIE; CK10
• Reactivity: Human, Cat
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
• Synonyms: Cytokeratin 10/13; Monoclonal Antibody; Cytokeratin 10/13 Mouse Monoclonal Antibody; BCIE; BIE; EHK; Keratin Type I Cytoskeletal 10; KRT10; CK13; K13; keratin type I cytoskeletal 13; Keratin-13; Krt1-13; KRT1; anti-KRT10 antibody

• Host: Mouse
• Reactivity: Human, Cat
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: SPM262
• Specificity: This antibody recognizes cytokeratin 10 (56.5kDa) and cytokeratin 13 (53kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this MAb serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 584

• Applicable Applications for anti-KRT10 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml); Western Blot (0.25-0.5ug/ml); Immunohistochemistry (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Cytoplasmic
• Immunogen: Cytoskeletal preparation extracted from human ectocervical epithelium
• Hu-Chromosome Location: 17q21.2
• Positive Control: A431, HeLa, MCF7 cells. Tonsil, esophageal or bladder carcinoma.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Tonsil stained with Cytokeratin 10/13 Monoclonal Antibody (SPM262))

References

Ivanyi D et. al. Journal of Pathology, 1989, 159:7-12.,Ivanyi, D., Minke, J. M., Hageman, C., Groeneveld, E., and van Doornewaard, G. (1992). Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells, Am J Vet Res 53, 304-14.,Ivanyi, D., Minke, J. M., Hageman, C., Groeneveld, E., van Doornewaard, G., and Misdorp, W. (1993). Cytokeratins as markers of initial stages of squamous metaplasia in feline mammary carcinomas, Am J Vet Res 54, 1095

NCBI and Uniprot Product Information

• NCBI GI #: 195972866
• NCBI GeneID: 3858
• NCBI Accession #: NP_000412.3
• NCBI GenBank Nucleotide #: NM_000421.3
• UniProt Accession #: P13645; Q14664; Q8N175
• Molecular Weight: 56.5kDa (CK10); 53kDa (CK13)
• NCBI Official Full Name: keratin, type I cytoskeletal 10
• NCBI Official Synonym Full Names: keratin 10
• NCBI Official Symbol: KRT10
• NCBI Official Synonym Symbols: BIE; EHK; K10; KPP; BCIE; CK10
• NCBI Protein Information: keratin, type I cytoskeletal 10
• UniProt Protein Name: Keratin, type I cytoskeletal 10
• Protein Family: Keratin
• UniProt Gene Name: KRT10
• UniProt Synonym Gene Names: KPP; CK-10; K10
• UniProt Entry Name: K1C10_HUMAN

NCBI Description

This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008]

Uniprot Description

K10: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K1.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: cytoplasm; extracellular space; membrane; nucleus

Biological Process: keratinocyte differentiation

Disease: Epidermolytic Hyperkeratosis; Erythroderma, Ichthyosiform, Congenital Reticular; Ichthyosis, Cyclic, With Epidermolytic Hyperkeratosis