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Western Blot (WB) (Western Blot: Sample: Rat Heart lysate; Primary Ab: 2ug/ml Mouse Anti-Human CYCS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Mouse anti-Human Cytochrome C, Somatic (CYCS) Monoclonal Antibody | anti-CYCS antibody

Monoclonal Antibody to Cytochrome C, Somatic (CYCS)

Gene Names
CYCS; CYC; HCS; THC4
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Protein A + Protein G affinity chromatography
Synonyms
Cytochrome C; Somatic (CYCS); Monoclonal Antibody; Monoclonal Antibody to Cytochrome C; Cyt-C; HCS; CYC; CytC; anti-CYCS antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a Kappa
Clone Number
C2
Specificity
The antibody is a mouse monoclonal antibody raised against CYCS. It has been selected for its ability to recognize CYCS in immunohistochemical staining and western blotting.
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Liquid
Concentration
1mg/mL (varies by lot)
Sequence Length
105
Applicable Applications for anti-CYCS antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
Western Blot: 0.5-5ug/mL
Immunohistochemistry: 5-30ug/mL
Immunocytochemistry: 5-30ug/mL
Immunogen
Recombinant Cytochrome C, Somatic (CYCS)
Cross Reactivity
Human, Rat
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Rat Heart lysate; Primary Ab: 2ug/ml Mouse Anti-Human CYCS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Rat Heart lysate; Primary Ab: 2ug/ml Mouse Anti-Human CYCS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant CYCS, Human.)

Western Blot (WB) (Western Blot: Sample: Recombinant CYCS, Human.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
11,749 Da
NCBI Official Full Name
cytochrome c
NCBI Official Synonym Full Names
cytochrome c, somatic
NCBI Official Symbol
CYCS
NCBI Official Synonym Symbols
CYC; HCS; THC4
NCBI Protein Information
cytochrome c
UniProt Protein Name
Cytochrome c
Protein Family
UniProt Gene Name
CYCS
UniProt Synonym Gene Names
CYC

NCBI Description

This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]

Uniprot Description

CYCS: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Belongs to the cytochrome c family.

Protein type: Apoptosis; Mitochondrial; Oxidoreductase; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 7p15.3

Cellular Component: cytosol; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrion; nucleus; protein phosphatase type 2A complex

Molecular Function: electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity; heme binding; metal ion binding; protein binding; protein serine/threonine phosphatase activity

Biological Process: activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c; cellular respiration; mitochondrial electron transport, cytochrome c to oxygen; mitochondrial electron transport, ubiquinol to cytochrome c; mitochondrion organization; protein amino acid dephosphorylation

Disease: Thrombocytopenia 4

Research Articles on CYCS

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Product Notes

The CYCS cycs (Catalog #AAA2090600) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Cytochrome C, Somatic (CYCS) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome C, Somatic (CYCS) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western Blot: 0.5-5ug/mL Immunohistochemistry: 5-30ug/mL Immunocytochemistry: 5-30ug/mL. Researchers should empirically determine the suitability of the CYCS cycs for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome C, Somatic (CYCS), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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