Mouse CYTOCHROME B245 Monoclonal Antibody | anti-CYBA antibody

MOUSE ANTI HUMAN CYTOCHROME B245 LIGHT CHAIN

Cat. Num. AAA219681

• Gene Names: CYBA; p22-PHOX
• Applications: Immunohistochemistry, ELISA, Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Synonyms: CYTOCHROME B245; Monoclonal Antibody; MOUSE ANTI HUMAN CYTOCHROME B245 LIGHT CHAIN; anti-CYBA antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: CS9
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 195

• Applicable Applications for anti-CYBA antibody: Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS)*, Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.; Western Blot: This item detects a band of approximately 19kDa in neutrophil membrane extracts; Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/100; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Immunogen: Cytochrome B245 solubilised in octyl-beta-gluycopyranoside; Fusion Partners
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain:Alexa Fluor ?488 (MBS219621A488). Permeabilised with Leucoperm)

Testing Data

(Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain:FITC (MBS219708). Permeabilised with Leucoperm)

Testing Data

(Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain:Alexa Fluor ?647 (MBS219621A647). Permeabilised with Leucoperm)

Testing Data

(Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain (MBS219621). Permeabilised with Leucoperm)

Related Product Information for anti-CYBA antibody

Mouse anti Human Cytochrome B245 Light Chain antibody, clone CS9 recognizes the light chain of cytochrome B245, also known as p22phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterised by severe recurrent infections. The light chain (p22phox), along with the heavy chain (gp91phox) form cytochrome B245 (also known as flavocytochome b, or Cyt b), which is always membrane-resident. Upon the appropriate stimulus, other components of NADPH oxidase (p47phox, p40phox, p67phox and Rac2) rapidly translocate from the cytosol to the cell membrane to form the complete enzyme complex. Cytochrome B245 appears to serve as a scaffold for the assembly of the other cytosolic units of the oxidase and to provide a transmembrane pathway for electrons. The core region of the CS9 epitope is amino acids 165 - 169 (KKPSE), a cytosolic epitope on p22phox not accessible on intact neutrophils. Mouse anti Human Cytochrome B245 Light Chain antibody, clone CS9 also has an inhibitory effect on NADPH oxidase activation.

NCBI and Uniprot Product Information

• NCBI GI #: 68509914
• NCBI GeneID: 1535
• NCBI Accession #: NP_000092.2
• NCBI GenBank Nucleotide #: NM_000101.3
• UniProt Accession #: P13498; Q14090; Q9BR72
• Molecular Weight: 21,013 Da
• NCBI Official Full Name: cytochrome b-245 light chain
• NCBI Official Synonym Full Names: cytochrome b-245, alpha polypeptide
• NCBI Official Symbol: CYBA
• NCBI Official Synonym Symbols: p22-PHOX
• NCBI Protein Information: cytochrome b-245 light chain; cytochrome b light chain; cytochrome b(558) alpha chain; cytochrome b(558) alpha-subunit; cytochrome b, alpha polypeptide; cytochrome b558 subunit alpha; flavocytochrome b-558 alpha polypeptide; neutrophil cytochrome b 22 kDa polypeptide; p22 phagocyte B-cytochrome; p22phox; superoxide-generating NADPH oxidase light chain subunit
• UniProt Protein Name: Cytochrome b-245 light chain
• Protein Family: Cytochrome
• UniProt Gene Name: CYBA
• UniProt Synonym Gene Names: p22phox
• UniProt Entry Name: CY24A_HUMAN

NCBI Description

Cytochrome b is comprised of a light chain (alpha) and a heavy chain (beta). This gene encodes the light, alpha subunit which has been proposed as a primary component of the microbicidal oxidase system of phagocytes. Mutations in this gene are associated with autosomal recessive chronic granulomatous disease (CGD), that is characterized by the failure of activated phagocytes to generate superoxide, which is important for the microbicidal activity of these cells. [provided by RefSeq, Jul 2008]

Uniprot Description

CYBA: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide. Defects in CYBA are a cause of chronic granulomatous disease autosomal recessive cytochrome-b-negative (ARCGD). Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. Belongs to the p22phox family.

Protein type: Oxidoreductase; Membrane protein, integral; EC 1.-.-.-

Chromosomal Location of Human Ortholog: 16q24

Cellular Component: Golgi apparatus; phagocytic vesicle membrane; membrane; mitochondrion; cell soma; dendrite; apical plasma membrane; plasma membrane; endosome; NADPH oxidase complex; secretory granule

Molecular Function: protein binding; electron carrier activity; protein heterodimerization activity; metal ion binding; superoxide-generating NADPH oxidase activity; heme binding; SH3 domain binding

Biological Process: response to drug; respiratory burst; response to nutrient levels; interaction with host; angiotensin-mediated regulation of glomerular filtration; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; positive regulation of cell growth; regulation of release of sequestered calcium ion into cytosol; antigen processing and presentation of peptide antigen via MHC class I; cytochrome complex assembly; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; positive regulation of endothelial cell proliferation; smooth muscle hypertrophy; inflammatory response; vascular endothelial growth factor receptor signaling pathway; superoxide release; hydrogen peroxide biosynthetic process

Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-negative