Mouse anti-Human, Mouse Cystic Fibrosis Transmembrane Conductance Regulator Monoclonal Antibody | anti-CFTR antibody

Cystic Fibrosis Transmembrane Conductance Regulator (CFTR, ABCC7)

Cat. Num. AAA608624

• Gene Names: CFTR; CF; MRP7; ABC35; ABCC7; CFTR/MRP; TNR-CFTR; dJ760C5.1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Ascites; Ascites
• Synonyms: Cystic Fibrosis Transmembrane Conductance Regulator; Monoclonal Antibody; Cystic Fibrosis Transmembrane Conductance Regulator (CFTR; ABCC7); Anti -Cystic Fibrosis Transmembrane Conductance Regulator (CFTR; anti-CFTR antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgM
• Clone Number: 4i327
• Specificity: Recognizes human CTFR. Also detects one or more immunologically related proteins in murine cell line Heb7a that does not contain CFTR mRNA. Can also be used to inhibit the epithelial uptake of S. typhi in some mouse cell lines. The peptide sequence is highly conserved in mouse, sheep, bovine and Xenopus laevis. Species Crossreactivity: mouse
• Purity/Purification: Ascites; Ascites
• Form/Format: Supplied as a liquid, 0.05% sodium azide.

• Applicable Applications for anti-CFTR antibody: Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, Flow cytometry, Immunoprecipitation and Western Blot.; Dilution: Immunofluorescence: 1:500; Immunofluorescence staining of CFTR in mouse epithelial cells results in cell surface staining, consistent with localization at the plasma membrane.; Western Blot: 1:500; Detects a single ~170kD protein representing CFTR in T84 whole cell extract.
• Immunogen: Synthetic peptide corresponding to aa103-117, RIIASYDPDNKEER in the first extracellular loop of human and rabbit CFTR. Cellular Localization: Membrane; multi-pass membrane protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Immunohistochemistry (paraffin) analysis in normal biopsies of deparaffinized human tonsil tissue using MBS608624.)

Immunohistochemistry (IHC)

(Immunohistochemistry (paraffin) analysis in cancer biopsies of deparaffinized human colon carcinoma tissue using MBS608624.)

Immunohistochemistry (IHC)

(Immunohistochemistry (paraffin) analysis in normal biopsies of deparaffinized human pancreas tissue using MBS608624.)

Immunocytochemistry (ICC)

(Immunofluorescence/Immunocytochemistry: CFTR staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown using MBS608624. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CFTR at a dilution of 1:100-1:200 over night at 4ºC, washed with PBS and incubated with a DyLight-488 conjugated.)

Related Product Information for anti-CFTR antibody

Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance factor, a cAMP regulated chloride channel. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 (delta F508) which results in abnormal chloride transport. Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is. One possible explanation is that Salmonella typhi has been shown to use CFTR to enter intestinal epithelial cells and that delta F508 heterozygote and homozygote mice showed 86% and 100% reductions in S. typhi intestinal submucosal uptake.

Product Categories/Family for anti-CFTR antibody

Antibodies; Abs to Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 90421313
• NCBI GeneID: 1080
• NCBI Accession #: NP_000483.3
• NCBI GenBank Nucleotide #: NM_000492.3
• UniProt Accession #: P13569; Q20BG8; Q20BH2; Q2I0A1; Q2I102; Q16049; Q20BH0; Q20BI4; Q20BI6; Q20BJ8; Q2I0A3; Q2I0A9; Q5I6F9; Q5I6N4
• Molecular Weight: 168,142 Da
• NCBI Official Full Name: cystic fibrosis transmembrane conductance regulator
• NCBI Official Synonym Full Names: cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
• NCBI Official Symbol: CFTR
• NCBI Official Synonym Symbols: CF; MRP7; ABC35; ABCC7; CFTR/MRP; TNR-CFTR; dJ760C5.1
• NCBI Protein Information: cystic fibrosis transmembrane conductance regulator; OTTHUMP00000024694; OTTHUMP00000196524; cAMP-dependent chloride channel; channel conductance-controlling ATPase; ATP-binding cassette sub-family C member 7; ATP-binding cassette transporter sub-family C member 7
• UniProt Protein Name: Cystic fibrosis transmembrane conductance regulator
• Protein Family: Cystic fibrosis transmembrane conductance regulator
• UniProt Gene Name: CFTR
• UniProt Synonym Gene Names: ABCC7; CFTR
• UniProt Entry Name: CFTR_HUMAN

NCBI Description

This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily that is involved in multi-drug resistance. The encoded protein functions as a chloride channel and controls the regulation of other transport pathways. Mutations in this gene are associated with the autosomal recessive disorders cystic fibrosis and congenital bilateral aplasia of the vas deferens. Alternatively spliced transcript variants have been described, many of which result from mutations in this gene. [provided by RefSeq]

Uniprot Description

CFTR: a member of the MRP subfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. Functions as a chloride channel and controls the regulation of other transport pathways. Mutations have been observed in patients with the autosomal recessive disorders cystic fibrosis (CF) and congenital bilateral aplasia of the vas deferens (CBAVD). Alternative splice

Protein type: Transporter, ABC family; Channel, chloride; Hydrolase; Membrane protein, multi-pass; Transporter; Membrane protein, integral; EC 3.6.3.49

Chromosomal Location of Human Ortholog: 7q31.2

Cellular Component: recycling endosome; microvillus; protein complex; cytoplasmic vesicle membrane; cell surface; basolateral plasma membrane; early endosome membrane; cytoplasm; early endosome; apical plasma membrane; plasma membrane

Molecular Function: bicarbonate transmembrane transporter activity; protein binding; chloride channel activity; enzyme binding; chloride channel inhibitor activity; chloride transmembrane transporter activity; ATP-binding and phosphorylation-dependent chloride channel activity; channel-conductance-controlling ATPase activity; ATP binding; PDZ domain binding

Biological Process: intracellular pH elevation; response to drug; response to peptide hormone stimulus; cholesterol transport; iodide transport; transepithelial chloride transport; water transport; membrane hyperpolarization; respiratory gaseous exchange; positive regulation of vasodilation; vasodilation; cholesterol biosynthetic process; cellular response to hormone stimulus; transport; bicarbonate transport; response to estrogen stimulus; response to cytokine stimulus; transmembrane transport; sperm capacitation; lung development

Disease: Vas Deferens, Congenital Bilateral Aplasia Of; Bronchiectasis With Or Without Elevated Sweat Chloride 1; Cystic Fibrosis; Pancreatitis, Hereditary