Mouse anti-Human Cystatin S Monoclonal Antibody | anti-CST4 antibody

Anti-Cystatin S Antibody, Mouse Monoclonal

Cat. Num. AAA8105402

• Reactivity: Human
• Applications: ELISA, ELISA
• Purity: Protein A
• Synonyms: Cystatin S; Monoclonal Antibody; Anti-Cystatin S Antibody; Mouse Monoclonal; Cystatin S/CST4 Antibody; Mouse MAb; cystatin S; Anti-CST4 Antibody; Anti-MGC71923 Antibody; anti-CST4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone Number: 9E2E2
• Specificity: Human Cystatin S/CST4.; No cross-reactivity in ELISA with Human cell lysate (293 cell line)
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CST4 antibody: ELISA (EIA), ELISA (EIA) (Cap)
• Application Notes: ELISA: 1:1000-1:2000; ELISA (Cap): 1:250-1:2000; ELISA (Cap): This antibody will detect Human Cystatin S/CST4 in ELISA pair set.; In a sandwich ELISA, it can be used as capture antibody when paired with.
• Immunogen: Recombinant Human Cystatin S/CST4 Protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Cystatin S/CST4 (rh Cystatin S/CST4). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-CST4 antibody

Cystatin-S, also known as Cystatin-4, Salivary acidic protein 1, Cystatin-SA-III and CST4, is a secreted protein which belongs to thecystatin family. Cystatin-4/CST4 is expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). It is also expressed in saliva, tears, urine and seminal fluid. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. Cystatin-4/CST4 strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively. Cystatin-4/CST4 is an S-type cystatin, based on its high level of expression in saliva, tears and seminal plasma. The specific role in these fluids is unclear but antibacterial and antiviral activity is present, consistent with a protective function.

References

Bobek L.A., et al., (1991), Human salivary cystatin S. Cloning, sequence analysis, hybridization in situ and immunocytochemistry. Biochem. J. 278:627-635.Saitoh E., et al., (1992), Characterization of two members (CST4 and CST5) of the cystatin gene family and molecular evolution of cystatin genes.Agents Actions 38:340-348.Deloukas P., et al., (2001), The DNA sequence and comparative analysis of human chromosome 20.Nature 414:865-871.

NCBI and Uniprot Product Information

• NCBI GI #: 4503109
• NCBI GeneID: 1472
• NCBI Accession #: NP_001890.1
• NCBI GenBank Nucleotide #: NM_001899.2
• UniProt Accession #: P01036; Q9UBI5; Q9UCS9
• Molecular Weight: 16,214 Da
• NCBI Official Full Name: cystatin-S
• NCBI Official Synonym Full Names: cystatin S
• NCBI Official Symbol: CST4
• NCBI Protein Information: cystatin-S; cystatin 4; cystatin-4; cystatin-SA-III; salivary acidic protein 1
• UniProt Protein Name: Cystatin-S
• Protein Family: Cystatin
• UniProt Gene Name: CST4
• UniProt Entry Name: CYTS_HUMAN

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a type 2 salivary cysteine peptidase inhibitor. The protein is an S-type cystatin, based on its high level of expression in saliva, tears and seminal plasma. The specific role in these fluids is unclear but antibacterial and antiviral activity is present, consistent with a protective function. [provided by RefSeq, Jul 2008]

Uniprot Description

CST4: This protein strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively. Belongs to the cystatin family.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: extracellular space

Molecular Function: cysteine protease inhibitor activity

Biological Process: negative regulation of proteolysis; retinal homeostasis; detection of chemical stimulus involved in sensory perception of bitter taste