Mouse anti-Human Cystatin C Monoclonal Antibody | anti-CST3 antibody

Cystatin C Mouse Monoclonal Antibody(5A2)

Cat. Num. AAA9417552

• Gene Names: CST3; ARMD11; HEL-S-2
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Synonyms: Cystatin C; Monoclonal Antibody; Cystatin C Mouse Monoclonal Antibody(5A2); CST3; anti-CST3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5A2
• Specificity: Cystatin C Mouse Monoclonal Antibody(5A2) detects endogenous levels of CST3
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 146

• Applicable Applications for anti-CST3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western Blot: 1:1000-1:2000; Immunohistochemistry: 1:100-1:200
• Immunogen: Recombinant Protein
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western Blot analysis of Cystatin C protein using antibody diluted at 1:1000)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using Cystatin C Mouse mAb diluted at 1:200.)

Product Categories/Family for anti-CST3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 755738
• NCBI GeneID: 1471
• UniProt Accession #: P01034; Q6FGW9; B2R5J9; D3DW42
• Molecular Weight: 15,799 Da
• NCBI Official Full Name: cystatin C
• NCBI Official Synonym Full Names: cystatin C
• NCBI Official Symbol: CST3
• NCBI Official Synonym Symbols: ARMD11; HEL-S-2
• NCBI Protein Information: cystatin-C
• UniProt Protein Name: Cystatin-C
• Protein Family: Cystatin
• UniProt Gene Name: CST3
• UniProt Entry Name: CYTC_HUMAN

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associated with amyloid angiopathy. Expression of this protein in vascular wall smooth muscle cells is severely reduced in both atherosclerotic and aneurysmal aortic lesions, establishing its role in vascular disease. In addition, this protein has been shown to have an antimicrobial function, inhibiting the replication of herpes simplex virus. Alternative splicing results in multiple transcript variants encoding a single protein. [provided by RefSeq, Nov 2014]

Uniprot Description

CST3: As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Defects in CST3 are the cause of amyloidosis type 6 (AMYL6); also known as hereditary cerebral hemorrhage with amyloidosis (HCHWA), cerebral amyloid angiopathy (CAA) or cerebroarterial amyloidosis Icelandic type. AMYL6 is a hereditary generalized amyloidosis due to cystatin C amyloid deposition. Cystatin C amyloid accumulates in the walls of arteries, arterioles, and sometimes capillaries and veins of the brain, and in various organs including lymphoid tissue, spleen, salivary glands, and seminal vesicles. Amyloid deposition in the cerebral vessels results in cerebral amyloid angiopathy, cerebral hemorrhage and premature stroke. Cystatin C levels in the cerebrospinal fluid are abnormally low. Genetic variations in CST3 are associated with age- related macular degeneration type 11 (ARMD11). ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. Belongs to the cystatin family.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: extracellular region; extracellular space

Molecular Function: beta-amyloid binding; cysteine protease inhibitor activity; endopeptidase inhibitor activity; identical protein binding; protease binding; protein binding

Biological Process: cellular protein metabolic process; defense response; fibril organization and biogenesis; negative regulation of peptidase activity; negative regulation of proteolysis; regulation of tissue remodeling

Disease: Cerebral Amyloid Angiopathy, Cst3-related; Macular Degeneration, Age-related, 11