Mouse Cyclin D1,2 Monoclonal Antibody | anti-CCND1 antibody

Cyclin D1,2 (AP)

Cat. Num. AAA6241525

• Gene Names: CCND1; BCL1; PRAD1; U21B31; D11S287E
• Reactivity: Hamster, Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot, Immunoblot, Western Blot
• Purity: Purified by Protein A Affinity Chromatography
• Synonyms: Cyclin D1; 2; Monoclonal Antibody; 2 (AP); anti-CCND1 antibody

• Host: Mouse
• Reactivity: Hamster, Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 0.T.25
• Specificity: Recognizes p36 cyclin D1 and crossreacts with p34 cyclin D2, which has a highly homologous epitope. No crossreactivity with cyclin A, cyclin B or cyclin D3. Species crossreactivity: human, mouse, rat and hamster.
• Purity/Purification: Purified by Protein A Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-CCND1 antibody: Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB), Immunoblot
• Application Notes: IHC-P: IHC: Reported to detect cyclin D1 and D2 in paraffin-embedded sections using high energy antigen retrieval (10mM citrate buffer, pH 6.0, microwaved 3-5 minutes, three times).; IP: 5ug immunoprecipitates cyclin D1 and D2 from 500ug of A431 RIPA lysate.; Applications are based on unconjugated antibody.; Immunoblot Analysis: 0.5-2ug/ml detects cyclin D1 and D2 in RIPA lysates from human A431 cells. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed (2ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
• Immunogen: Recombinant human Cyclin D1 expressed in E. coli.
• Conjugate: AP
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-CCND1 antibody

Antibodies; Cyclin

References

1. Banno, S., et al., Japanese J. Cancer Res. 85: 918-926, 1994.

NCBI and Uniprot Product Information

• NCBI GI #: 16950655
• NCBI GeneID: 595
• NCBI Accession #: NP_444284.1
• NCBI GenBank Nucleotide #: NM_053056.2
• UniProt Accession #: P24385; Q6LEF0
• Molecular Weight: 33,729 Da
• NCBI Official Full Name: G1/S-specific cyclin-D1
• NCBI Official Synonym Full Names: cyclin D1
• NCBI Official Symbol: CCND1
• NCBI Official Synonym Symbols: BCL1; PRAD1; U21B31; D11S287E
• NCBI Protein Information: G1/S-specific cyclin-D1; BCL-1 oncogene; PRAD1 oncogene; B-cell CLL/lymphoma 1; B-cell lymphoma 1 protein
• UniProt Protein Name: G1/S-specific cyclin-D1
• Protein Family: G1/S-specific cyclin
• UniProt Gene Name: CCND1
• UniProt Synonym Gene Names: BCL1; PRAD1; BCL-1
• UniProt Entry Name: CCND1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

CCND1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb.

Protein type: Cell cycle regulation; Nuclear receptor co-regulator; Activator; Oncoprotein

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; tight junction; membrane; transcriptional repressor complex; cyclin-dependent protein kinase holoenzyme complex; intracellular; nucleus; cytosol

Molecular Function: protein binding; enzyme binding; histone deacetylase binding; protein complex binding; cyclin-dependent protein kinase regulator activity; transcription corepressor activity; protein kinase binding; transcription factor binding; protein kinase activity

Biological Process: lactation; G1 DNA damage checkpoint; fat cell differentiation; establishment and/or maintenance of chromatin architecture; re-entry into mitotic cell cycle; positive regulation of cyclin-dependent protein kinase activity; negative regulation of Wnt receptor signaling pathway; positive regulation of mammary gland epithelial cell proliferation; negative regulation of epithelial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; protein amino acid phosphorylation; response to magnesium ion; response to vitamin E; Leydig cell differentiation; response to iron ion; response to X-ray; response to corticosterone stimulus; response to drug; Notch signaling pathway; organ regeneration; transcription, DNA-dependent; unfolded protein response; mammary gland epithelial cell proliferation; response to organic nitrogen; liver development; response to ethanol; cell division; response to estrogen stimulus; mitotic cell cycle; positive regulation of protein amino acid phosphorylation; response to calcium ion; response to DNA damage stimulus; G1/S transition of mitotic cell cycle

Disease: Von Hippel-lindau Syndrome; Myeloma, Multiple