ELISA (EIA)
(Sandwich ELISA analysis of human CXCL16 using Mouse anti Human CXCL16 as a capture reagent and biotinylated Mouse anti Human CXCL16 (MBS226133) as a detection reagent with recombinant human CXCL16 as the antigen. Detection was performed using H)
Mouse anti-Human CXCL16 Monoclonal Antibody | anti-CXCL16 antibody
Mouse anti Human CXCL16:Biotin
Gene Names
CXCL16; SRPSOX; CXCLG16; SR-PSOX
Reactivity
Human
Applications
ELISA
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
CXCL16; Monoclonal Antibody; Mouse anti Human CXCL16:Biotin; CXCL16 antibody | C19-5F9; anti-CXCL16 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
C19-5F9
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Phosphate buffered saline
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Purified IgG - liquid
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Purified IgG - liquid
Concentration
IgG concentration 0.1mg/ml (varies by lot)
Applicable Applications for anti-CXCL16 antibody
ELISA (EIA)
Application Notes
ELISA: 1.0ug/ml
Immunogen
E Coli derived recombinant human full length CXCL16 (Asn49-Pro137)
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
Conjugation
Biotin
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
ELISA (EIA)
(Sandwich ELISA analysis of human CXCL16 using Mouse anti Human CXCL16 as a capture reagent and biotinylated Mouse anti Human CXCL16 (MBS226133) as a detection reagent with recombinant human CXCL16 as the antigen. Detection was performed using H)
ELISA (EIA)
(Sandwich ELISA analysis of human CXCL16 using Mouse anti Human CXCL16 as a capture reagent and biotinylated Mouse anti Human CXCL16 (MBS226133) as a detection reagent with recombinant human CXCL16 as the antigen. Detection was performed using H)
Related Product Information for anti-CXCL16 antibody
Mouse anti Human CXCL16 antibody, clone C19-5F9, recognizes C-X-C motif Chemokine 16 (CXCL16). Unlike most chemokines, CXCL16 is a membrane bound ligand anchored through transmembrane domains. Although a soluble form of CXCL16 is generated through proteolytic cleavage. Transmembrane CXCL16 is mainly expressed on macrophages, dendritic cells, B cells and various tumor cells. In this form, it acts as an adhesion molecule though the binding of its receptor CXCR6 expressed on other cells including T cells. Transmembrane CXCL16 also binds gram positive and gram negative bacteria, playing a role in innate and adaptive immune responses (Shimaoka et al. 2003).
Soluble CXCL16 has chemoattractant activity, and elicits this response through the receptor CXCR6.There is also mounting evidence suggesting an additional role in tumor progression. With a number of studies suggesting CXCL16 as a biomarker for the evaluation of some tumor type prognoses (Richardsen et al. 2015, Shibata et al. 2020, Gooden et al. 2014).
The biotinylated Mouse anti Human CXCL16 antibody, clone C19-5F9 can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human CXCL16 antibody, clone B06-3H6 as the capture antibody.
Soluble CXCL16 has chemoattractant activity, and elicits this response through the receptor CXCR6.There is also mounting evidence suggesting an additional role in tumor progression. With a number of studies suggesting CXCL16 as a biomarker for the evaluation of some tumor type prognoses (Richardsen et al. 2015, Shibata et al. 2020, Gooden et al. 2014).
The biotinylated Mouse anti Human CXCL16 antibody, clone C19-5F9 can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human CXCL16 antibody, clone B06-3H6 as the capture antibody.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,579 Da
NCBI Official Full Name
C-X-C motif chemokine 16
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 16
NCBI Official Symbol
CXCL16
NCBI Official Synonym Symbols
SRPSOX; CXCLG16; SR-PSOX
NCBI Protein Information
C-X-C motif chemokine 16; CXC chemokine ligand 16; small-inducible cytokine B16; transmembrane chemokine CXCL16; scavenger receptor for phosphatidylserine and oxidized low density lipoprotein
UniProt Protein Name
C-X-C motif chemokine 16
Protein Family
UniProt Gene Name
CXCL16
UniProt Synonym Gene Names
SCYB16; SRPSOX; SR-PSOX
UniProt Entry Name
CXL16_HUMAN
Uniprot Description
CXCL16: Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis. Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo. Belongs to the intercrine alpha (chemokine CxC) family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 17p13
Cellular Component: extracellular space; membrane; extracellular region; plasma membrane; integral to membrane
Molecular Function: low-density lipoprotein receptor activity; chemokine activity; scavenger receptor activity
Biological Process: receptor-mediated endocytosis; response to cytokine stimulus; chemotaxis; positive regulation of cell growth; positive regulation of cell migration
Research Articles on CXCL16
Similar Products
Product Notes
The CXCL16 cxcl16 (Catalog #AAA226133) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Human CXCL16:Biotin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CXCL16 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). ELISA: 1.0ug/ml. Researchers should empirically determine the suitability of the CXCL16 cxcl16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CXCL16, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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