Mouse CUG-Binding Protein 1 Ribonucleoprotein Monoclonal Antibody | anti-CELF1 antibody

CUG-Binding Protein 1 Ribonucleoprotein (CUG-BPI, Cytidine Uridine Guanosine Binding Protein 1)

Cat. Num. AAA600774

• Gene Names: CELF1; CUGBP; NAB50; NAPOR; CUG-BP; CUGBP1; hNab50; BRUNOL2; EDEN-BP
• Reactivity: Bovine, Human, Mouse, Porcine, Rabbit, Rat
• Applications: Western Blot, Immunoprecipitation, Immunocytochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: CUG-Binding Protein 1 Ribonucleoprotein; Monoclonal Antibody; CUG-Binding Protein 1 Ribonucleoprotein (CUG-BPI; Cytidine Uridine Guanosine Binding Protein 1); Anti -CUG-Binding Protein 1 Ribonucleoprotein (CUG-BPI; anti-CELF1 antibody

• Host: Mouse
• Reactivity: Bovine, Human, Mouse, Porcine, Rabbit, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 1.T.9
• Specificity: Recognizes human CUG-BP1, Mr 50kD. Species Crossreactivity: Reported to crossreact with mouse, rat, bovine, porcine and rabbit
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.6, 0.07% sodium azide, before the addition of glycerol to 30%

• Applicable Applications for anti-CELF1 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Supershift Assay.; Dilution: Western Blot Analysis: 0.1-2.0ug/ml detects CUG-BP1 in HeLa nuclear extract. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with C7948-50 (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.; Immunoprecipitation: Immunoprecipitates CUG-BP1 from HeLa cell lysates; Immunocytochemistry: Nuclear staining in HeLa and myoblasts from normal patients.; Supershift: Supershifts CUG-BP1:DNA complexes in EMSA
• Immunogen: Full-length GST fusion protein corresponding to human CUG-BP1, also known as heterogeneous nuclear ribonucleoprotein (hnRNP) hNab50.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(WB: Hela nuclear extract probed with MBS600774 (0.5ug/ml).)

Product Categories/Family for anti-CELF1 antibody

Antibodies; Abs to Nuclear Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 5729794
• NCBI GeneID: 10658
• NCBI Accession #: NP_006551.1
• NCBI GenBank Nucleotide #: NM_006560.3
• UniProt Accession #: Q92879; Q4LE52; Q9NP83; Q9NR06; D3DQS0
• Molecular Weight: 52,063 Da
• NCBI Official Full Name: CUGBP Elav-like family member 1 isoform 1
• NCBI Official Synonym Full Names: CUGBP, Elav-like family member 1
• NCBI Official Symbol: CELF1
• NCBI Official Synonym Symbols: CUGBP; NAB50; NAPOR; CUG-BP; CUGBP1; hNab50; BRUNOL2; EDEN-BP
• NCBI Protein Information: CUGBP Elav-like family member 1; CELF-1; CUG-BP1; bruno-like 2; EDEN-BP homolog; OTTHUMP00000233203; OTTHUMP00000233204; OTTHUMP00000233205; OTTHUMP00000238034; OTTHUMP00000238035; OTTHUMP00000238036; bruno-like protein 2; CUG RNA-binding protein; deadenylation factor CUG-BP; RNA-binding protein BRUNOL-2; CUG-BP- and ETR-3-like factor 1; CUG triplet repeat RNA-binding protein 1; CUG triplet repeat, RNA binding protein 1; CUG triplet repeat, RNA-binding protein 1; embryo deadenylation element binding protein; 50 kDa nuclear polyadenylated RNA-binding protein; nuclear polyadenylated RNA-binding protein, 50-kD; embryo deadenylation element-binding protein homolog
• UniProt Protein Name: CUGBP Elav-like family member 1
• Protein Family: CUGBP Elav-like family
• UniProt Gene Name: CELF1
• UniProt Synonym Gene Names: BRUNOL2; CUGBP; CUGBP1; NAB50
• UniProt Entry Name: CELF1_HUMAN

NCBI Description

Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Uniprot Description

CELF1: RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre- mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver. Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis. Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the IR RNA. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver. Belongs to the CELF/BRUNOL family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA processing; Cell development/differentiation; RNA-binding

Chromosomal Location of Human Ortholog: 11p11

Cellular Component: nucleoplasm; membrane; cytoplasm; ribonucleoprotein complex; nucleus

Molecular Function: mRNA binding; protein binding; translation initiation factor binding; RNA binding; BRE binding; translation repressor activity, nucleic acid binding; nucleotide binding

Biological Process: mRNA splice site selection; embryonic development; regulation of RNA splicing; negative regulation of translation; positive regulation of multicellular organism growth; mRNA processing; spermatid development; germ cell development; RNA interference