Mouse anti-Human, Mouse CTH Monoclonal Antibody | anti-CTH antibody

CTH (Cystathionine gamma-lyase, Cysteine-protein Sulfhydrase, Gamma-cystathionase) (Biotin)

Cat. Num. AAA6141398

• Reactivity: Human, Mouse
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CTH; Monoclonal Antibody; CTH (Cystathionine gamma-lyase; Cysteine-protein Sulfhydrase; Gamma-cystathionase) (Biotin); anti-CTH antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: S51
• Specificity: Recognizes human CTH. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CTH antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 3ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-405 from human CTH (AAH15807) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MQEKDASSQGFLPHFQHFATQAIHVGQDPEQWTSRAVVPPISLSTTFKQGAPGQHSGFEYSRSGNPTRNCLEKAVAALDGAKYCLAFASGLAATVTITHLLKAGDQIICMDDVYGGTNRYFRQVASEFGLKISFVDCSKIKLLEAAITPETKLVWIETPTNPTQKVIDIEGCAHIVHKHGDIILVVDNTFMSPYFQRPLALGADISMYSATKYMNGRSDVVMGLVSVNCESLHNRLRFLQNSLGAVPSPIDCYLCNRGLKTLHVRMEKHFKNGMAVAQFLESNPWVEKVIYPGLPSHPQHELVKRQCTGCTGMVTFYIKGTLQHAEIFLKNLKLFTLAESLGGFESLAELPAIMTHASVLKNDRDVLGISDTLIRLSVGLEDEEDLLEDLDQALKAAHPPSGSHS
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (70.07kD).)

Western Blot (WB)

(CTH monoclonal antibody. Western Blot analysis of CTH expression in K-562.)

Western Blot (WB)

(Western Blot analysis of CTH expression in transfected 293T cell line by CTH monoclonal antibody. Lane 1: CTH transfected lysate (45kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to CTH on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged CTH is ~1ng/ml as a capture antibody.)

Product Categories/Family for anti-CTH antibody

Antibodies; Enzymes

References

1. An investigation of the mechanisms of hydrogen sulfide-induced vasorelaxation in rat middle cerebral arteries. Streeter E, Hart J, Badoer E.Naunyn Schmiedebergs Arch Pharmacol. 2012 Jul 17. 2. Characterization of Hydrogen Sulfide and Its Synthases, Cystathionine ?]-Synthase and Cystathionine ?^-Lyase, in Human Prostatic Tissue and Cells. Guo H, Gai JW, Wang Y, Jin HF, Du JB, Jin J.Urology. 2012 Feb;79(2):483.e1-5. 3. Glucocorticoids suppress cystathionine gamma-lyase expression and H(2)S production in lipopolysaccharide-treated macrophages. Zhu XY, Liu SJ, Liu YJ, Wang S, Ni X.Cell Mol Life Sci. 2010 Jan 10. 4. H2S contributes to the hepatic arterial buffer response and mediates vasorelaxation of the hepatic artery via activation of KATP channels. Siebert N, Cantre D, Eipel C, Vollmar B.Am J Physiol Gastrointest Liver Physiol. 2008 Dec;295(6):G1266-73. Epub 2008 Oct 30.

NCBI and Uniprot Product Information

• NCBI GI #: 34194108
• NCBI GeneID: 1491
• Molecular Weight: 41,260 Da
• NCBI Official Full Name: Homo sapiens cystathionase (cystathionine gamma-lyase), mRNA
• NCBI Official Synonym Full Names: cystathionine gamma-lyase
• NCBI Official Symbol: CTH
• NCBI Protein Information: cystathionine gamma-lyase
• Protein Family: Cystathionine gamma-lyase

NCBI Description

This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]