Mouse CTH Monoclonal Antibody | anti-CTH antibody

Mouse Monoclonal [clone 2D6] (IgG1) to Human CTH

Cat. Num. AAA249175

• Reactivity: Dog, Human, Monkey
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein A/G Purified
• Synonyms: CTH; Monoclonal Antibody; Mouse Monoclonal [clone 2D6] (IgG1) to Human CTH; Anti-CTH Antibody (clone 2D6) IHC-plus; Cysteine-protein sulfhydrase; Cysteine desulfhydrase; Cystathionine gamma-lyase; Homoserine dehydratase; Gamma-cystathionase; Homoserine deaminase; Human CTH; anti-CTH antibody

• Host: Mouse
• Reactivity: Dog, Human, Monkey
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2D6
• Specificity: Human CTH
• Purity/Purification: Protein A/G Purified
• Form/Format: PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
• Concentration: 0.9 mg/ml (varies by lot)
• Sequence Length: 405

• Applicable Applications for anti-CTH antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (1:200 - 1:500)
• Target Species: Human
• Immunogen Description: Full length human recombinant protein of human CTH (NP_001893) produced in HEK293T cell.
• Immunogen Type: Recombinant protein
• Immunogen: CTH antibody was raised against full length human recombinant protein of human CTH (NP_001893) produced in HEK293T cell.
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-CTH antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Western blot of extracts (35 ug) from 9 different cell lines by using anti-CTH monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).)

Western Blot (WB)

(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTH.)

NCBI and Uniprot Product Information

• NCBI GI #: 21361334
• NCBI GeneID: 1491
• NCBI Accession #: NP_001893.2
• NCBI GenBank Nucleotide #: NM_001902.5
• UniProt Accession #: P32929; Q53FB3; Q53Y79; Q9H4W7; Q9H4W8; B4E1R2; E9PDV0
• Molecular Weight: 41,260 Da
• NCBI Official Full Name: cystathionine gamma-lyase isoform 1
• NCBI Official Synonym Full Names: cystathionine gamma-lyase
• NCBI Official Symbol: CTH
• NCBI Protein Information: cystathionine gamma-lyase; gamma-cystathionase; homoserine deaminase; cysteine desulfhydrase; homoserine dehydratase; cysteine-protein sulfhydrase; cystathionase (cystathionine gamma-lyase)
• UniProt Protein Name: Cystathionine gamma-lyase
• Protein Family: Cystathionine gamma-lyase
• UniProt Gene Name: CTH
• UniProt Entry Name: CGL_HUMAN

NCBI Description

This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]

Uniprot Description

CTH: Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function. Defects in CTH are the cause of cystathioninuria (CSTNU). It is an autosomal recessive phenotype characterized by abnormal accumulation of plasma cystathionine, leading to increased urinary excretion. Belongs to the trans-sulfuration enzymes family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lyase; Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - glycine, serine and threonine; EC 4.4.1.1; Other Amino Acids Metabolism - selenoamino acid; Cell cycle regulation; Energy Metabolism - nitrogen

Chromosomal Location of Human Ortholog: 1p31.1

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: calmodulin binding; protein binding; cystathionine gamma-lyase activity; carbon-sulfur lyase activity; homocysteine desulfhydrase activity; pyridoxal phosphate binding

Biological Process: cysteine metabolic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; sulfur amino acid metabolic process; unfolded protein response; protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine; cysteine biosynthetic process; sulfur amino acid catabolic process; transsulfuration; activation of NF-kappaB transcription factor; protein homotetramerization

Disease: Cystathioninuria